Entamoeba Histolytica troph
Ave size
12-25um
Entamoeba Histolytica cyst
Ave size
12-18um
Entamoeba Histolytica troph
movement
Unidirectional
Rapid and progressive
Entamoeba Histolytica
Number of nuclei
Troph: 1
Cyst: 4
Entamoeba Histolytica
Karyosome
Troph: small, central w/ peripheral chromatin
Cyst: small, central
Entamoeba Histolytica
Peripheral chromatin
both Fine and evenly distributed
Entamoeba Histolytica
cytoplasm
Ground glass appearance
Finely granular
Entamoeba Histolytica
cytoplasmic inclusions
Troph: ingested RBC
Cyst: Chromatoidal Bars
Where excystation happens
Ileum
Flask shaped ulcer
Cecum ascending colon
Virulence factors of Flask Shaped Ulcer
Gal-lectin
Amoeba pore
Cysteine Proteinases
Bacillary dysentery
Vomitting common
Amebic dysentery
No vomitting
Entamoeba Histolytica
Diagnosis
DFS: NSS and perma stain (PVA or mPVA)
Robinsons culture
Concentration technique-FECT + PCR DNA amplification
Serological
Entamoeba Histolytica
treatment
Metronidazole (Flagyl)
Entamoeba Histolytica
complications
Abemic Liver Abcess
Extraintestinal Amebiasis
Ameboma
Entamoeba Coli
Size
Troph: 18-27um
Cyst: 12-25um
Entamoeba Coli
Motility
Non progressive
Blunt pseudopods
Entamoeba Coli
Nuclei
Troph: 1
Cysts: 1-8
Entamoeba Coli
Karyosome
Both Large, irregular shape, eccentric
Entamoeba Coli
Peripheral chromatin
Both unevenly distributed
Entamoeba Coli
Cystoplasm
Both coarse and granulated
Entamoeba Coli
Cytoplasmic inclusions
Troph:
Vacuole w/ bacteria
Yeast cell
(Dirty looking amoeba)
Cyst:
Diffuse glycogen
Thin chromatoidal bars
Entamoeba Hartmanni
Morphology
Similar to E. Histolytica
WITHOUT RBC
Entamoeba Hartmanni
Size
Troph: 5-15
Cyst: 7-9
Entamoeba Powlecki
Size
Troph: 8-25
Cyst: 10-20
Entamoeba Powlecki
Motility
Troph: Sluggish, unidirectional progressive
Entamoeba Powlecki
Nuclei
Both 1
Entamoeba Powlecki
Peripheral chromatin
Sausage like
Fine and evenly distributed
Entamoeba Powlecki
Cytoplasmic inclusions
Troph: Ingested bacteria and food particles
Cyst: chromatoidal bars
Endolimax nana
Size
Smallest
Troph: 7-10um
Cyst: 4-12um
Endolimax nana
Motility
Sluggish, non-progressive, blunt hyaline pseudopod
Endolimax nana
Nuclei
Troph: 1
Cyst: 1-4
Endolimax nana
Karyosome
Blot like
Endolimax nana
Peripheral chromatin
ABSENT
Endolimax nana
Cytoplasm
Granular and vacuolated
Endolimax nana
Cytoplasmic inclusions
Bacteria
Comma shaped chromatoidal bars
Iodamoeba butschlii
Size
Troph: 8-22um
Cyst: 8-12um
Iodamoeba butschlii
Motility and shape
Sluggish progressive
Triangular
Iodamoeba butschlii
Karyosome
Basket flowers shape in cysts
Iodamoeba butschlii
Cytoplasmic inclusions
Well defined glycogen mass
Bacteria
Yeast cell
Entamoeba gingivalis
Size
8-20
NO CYST
Entamoeba gingivalis
Motility
Active motility
Varying pesudopod
Entamoeba gingivalis
Nuclei
1
Entamoeba gingivalis
Karyosome
Centrally located
Entamoeba gingivalis
Cytiplasmic inclusions
Leukocytes
Ephithelial cells
Bacteria
Entamoeba gingivalis
Habitat
Gum line or tonsilar crypts
Entamoeba gingivalis
Transmitted by
Bronchial mucus
Entamoeba gingivalis
Isolated by
Bronchial mucus
Pulmonary abcess
Sputum
Intea urethral device
Naegleria fowleri
Size
Troph: 8-22
Cyst: 9-12
Flagellate: 7-15um
Naegleria fowleri
Peripheral chromatin
ABSENT
Naegleria fowleri
Specimen of choice
CSF, tissue and nasal discharge
Naegleria fowleri
Treatment
Amphotericin B or
Micronazole
Acanthamoeba
Size
Troph: 12-45um
Cyst: 8-25um
Acanthamoeba
Specimen of choice
CSF, brain tissue, corneal scrapings
Acanthamoeba
Stain
Calcoflour white