Ch. 16 Quiz Flashcards Preview

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Flashcards in Ch. 16 Quiz Deck (15)
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1
Q

What process is used to create transgenic animals?

A

Nuclear microinjection

2
Q

In the first transgenic animal experiment, which gene from rats was cloned?

A

Somatotropin

3
Q

According to the book, which protein is produced by transgenic goats?

A

Recombinant tissue plasminogen activator

4
Q

Why are embryonic stem cells important?

A

They can develop into any tissue in the body, including the germ line

5
Q

How can location effects of transgenes in animals be avoided?

A

All of the above

  • by placing LCR sequences in front of the transgene
  • by inclusion of insulator sequences on both ends of the transgene
  • by using natural transgenes instead of the cDNA version
  • by targeting the transgene to a specific location
6
Q

What is used by targeting vectors to insert transgenes at specific locations within the host gene?

A

Homologous recombination

7
Q

Which of the following systems can be used to control the expression of the transgene?

A

All of the above

  • a modified Lacl repressor system
  • the TetR recombinant promoter system
  • use of a heat shock promoter from Drosphila
  • the use of steroid receptors
8
Q

Why are Cre/loxP or Flp/FRT used in transgenic animals?

A

All of the above

  • activation of a transgene by removing blocking sequences flanked by the loxP or FRT sites
  • large-scale deletions and rearrangements of the chromosomes
  • removal of selectable markers that are no longer needed
  • creation of conditional knockout mutants
9
Q

What can be engineered to introduce transgenes into Drosphila?

A

P elements

10
Q

How can mosquito-borne diseases be controlled through biotechnology?

A

By genetically engineering mosquitoes that are resistant to the disease

11
Q

Which of the following is derived from bacteria and useful for generating transgenes or modulating gene expression?

A

phiC31 integrase

12
Q

Transgenic mosquitoes can be produced using a transposon from the cabbage looper called ___.

A

piggyBac

13
Q

Concerning transgenic mosquitoes, which of these is not a strategy for the control of disease spread?

A

All of the above are potential strategies.

  • overexpression of defensin A from the mosquito’s pen immune system
  • expression of phospholipase from bees
  • expression of a human antibody in mosquito saliva
  • production of lysozyme in the mosquito gut
14
Q

Transgenic…

A

Mosquitoes can limit the spread of diseases such as malaria and dengue

15
Q

Antisense RNA can be introduced as transgenes and used in ___.

A

Gene silencing