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Flashcards in Degenerate PCR Deck (3)
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1

What are degenerate primers?

A pool of primers that bind to all variations of the target sequence

2

How are the primers designed?

Use amino acids that are coded for by 2 codons (C, D, E, F, H, K, N, Y) or 1 codon (M, W) to estimate what the triplet codon sequence is

3

What is codon bias?

Organisms will use certain codons over others to make amino acids