Diagnostic Virology Flashcards Preview

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Flashcards in Diagnostic Virology Deck (65)
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1

Which is false?
A. Most viral infections are self-limiting and asymptomatic
B. Non-specific symptoms of viral diseases like fever can be
due to a number of viral agents
C. A particular virus can produce a number of signs and
symptoms
D. Antiviral drugs are ubiquitous

D

2

In diagnostic virology, turn around time for most laboratory methods is not timely enough. T or F?

T

3

In specimen collection, handling and transport, what are the three As one must consider?

Appropriateness of specimen
Adequacy of specimen
Adherence to universal precautions

4

Diff viruses, diff ideal specimens. Which is false?
A. for respiratory diseases: nasopharyngeal aspirates
are the best specimens
B. for systemic diseases: blood
C. for HSV1 (singaw): swab only
D. none of the above

C

For HSV1: swab for microscopy and rapid
diagnosis, obtained from the base of lesion; aspirate
for acute infection with vesicular lesion

5

Which virus has diagnosis process where the specimen better obtained in convalescent phase?

SARS coronavirus

6

In which two stages of disease is virus frequently detectable in test materials?

onset & acute phase

7

In which three stages of the disease is specific antibody demonstrable?

acute phase, recovery and convalescence

8

Which is false?
A)If viral specimen cannot be transported immediately, store at -70 deg C or liquid nitrogen temperature (-180 deg
C)
B) Do not freeze specimens
C) Generally, all solid specimens except blood are transported in viral transport medium
D) none of the above

C

stool dapat

9

What three things must be present in virus transport media?

antibiotics, buffered salt solution and proteinaceous substance

10

method which would involve antigen-antbody interactions

serology

11

One of the methods of antigen detection is direct examination. What is done here?

use of whole or parts of virion

12

All are direct exam methods for antigen detection except?
a. light and electron microscopy
b. cell culture
c. viral genome detection

B

13

Which is not a classical technique of antigen detection?
A. Western blot
B. Neutralization
C. Counter-immunoelectrophoresis
D. Complement fixation

A

14

Which is not a newer technique of antigen detection?
A. Western blot
B. Particle agglutination
C. Counter-immunoelectrophoresis
D. Radioimunoassay

C

15

Which is not a classical technique of antigen detection?
A. Immunofluorescence
B. RIBA
C. Counter-immunoelectrophoresis
D. Complement fixation

B

16

Which is not a newer technique of antigen detection?
A. Line immunoassay
B. RIBA
C. Enzyme linked immunoassay
D. Hemagglutination inhibition

D

17

How is a virus sample decontaminated? (3 steps.)

centrifugation
filtration
antibiotic usage

18

When Shaula isolated a certain virus, she noticed that there was a change in color, from pink to yellow. What does this mean?

There was an exchange of gases, signifying metabolism.

19

Which is false?
A. All viruses are culturable
B. From isolates, you may know the sequence and
pathogenesis of the virus.
C. Cells should generally be liquid in form for inoculation in
37 deg C, with 5% CO2.

A.All viruses are culturable
(not all! i.e. Hepatitis virus: use of
molecular techniques)

20

Which is false?
A. Plates are used for diagnosis
B. Flasks are used for propagation of cells
C. The worst cells are the newly propagated ones.

C

best sila

21

Which is not a purpose of animal inoculation?
A. To produce viruses which cannot be propagated in vivo
B. To study the pathogenesis of virus infections, e.g.
coxsackie viruses
C. To test vaccine safety
D. None of the above

A

in vitro dapat yan

22

Identify the cell in culture
o Cells are bipolar or multipolar
o Elongated shapes
o Grow attached to a substrate

fibroblastic

23

identify the cell in culture
o Polygonal in shape with more regular dimensions
o Grow attached to a substrate in discrete patches

epithelial-like

24

identify the cell in culture
o Cells are spherical in shape
o Usually grown in suspension without attaching to a
surface

lymphoblast-like

25

False about primary culture of cells
A.Directly from parent tissue
B.Have the same karyotype and chromosome number as
the original tissue
C.Long life span

C.

Short dapat

26

False about diploid cell line
A. from continuous cell line
B. at least 75% of the cells have the same karyotype as
the cells of the original tissue
C. can survive for about 20-50 subcultures

A

continuous cell line ang galing sa diploid cell line

27

False about continuous cell line
A. from diploid cell line
B. at least 75% of the cells have the same karyotype as
the cells of the original tissue
C. can survive for about 20-50 subcultures
D. from benign tissue

D

malignant dapat

28

Which is false?
A.Primary culture involves cells from the liver
B.Primary culture involves fibroblast-like or
epithelial-like
C.Influenza virus is cultured in primary culture

A

kidney dapat

29

Which is false?
A. In continuous cell lines, Herpes Simplex virus and Varicella Zoster virus can be cultured.
B. Diploid cell lines are fibroblast in nature.
C. none of the above

A

diploid dapat

30

diploid cell line: __ from human kidney or
lung fibroblasts

WI-38 and MRC-5