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Flashcards in DNA Synthesis Deck (14)
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1
Q

The daughter DNA strand is synthesized in which direction?

A

5’–>3’

2
Q

The template strand of DNA is read in which direction during synthesis of a daughter strand?

A

3’–>5’

3
Q

DNA Pol requires what in order to replicate DNA?

A

Free 3’ –OH

4
Q

This enzyme adds primers to DNA for replication

A

Primase (RNA primer)

5
Q

Function of telemerase

A

Extend telomeres using reverse transcription

6
Q

Prokaryotic DNA replication initiation

A

Binding of DnaA protein at point of orgin OriC, proceeds bidirectionally

7
Q

This enzyme adds RNA primers to DNA strand in prokaryotic replication

A

DNA primase

8
Q

This DNA Pol fills in the gaps left by Okazaki fragments

A

Pol I

9
Q

Function of clamp proteins (prokaryotic)

A

Hold DNA Pol onto template strand

10
Q

DNA Pol that has “proofreading” function

A

DNA Pol III

11
Q

DNA synthesis can only occur during what time of the eukaryotic cell cycle?

A

S phase

12
Q

What is the major DNA Pol in eukaryotes?

A

Pol delta

13
Q

How long are Okazaki fragments in eukaryotes?

A

~200 bp

14
Q

Two functions of eukaryotic topoisomerases

A
  1. Relieve tension (Topo I)

2. Untangle DNA (Topo II)–>cancer target, only in proliferating cells