Functional genomics Flashcards Preview

BIO2030 Biotechnology > Functional genomics > Flashcards

Flashcards in Functional genomics Deck (6)
Loading flashcards...
1
Q

What is functional genomics?

A

Understanding biological functions through the use of genomic/transcriptomic data
Investigation of cell functions
Assigns functions to genes

2
Q

What are some methods in transcriptomics?

A
Northern blots
in situ hybridisation 
RT-qPCR
digital droplet PCR
Microarray
RNA-seq
3
Q

What are some methods in proteomics?

A
2DE-MS
LC/MS
Metalloproteomics
Phosphoproteomics
Structural genomics
4
Q

What is the transcriptome?

A

The set of all the mRNA in a cell

5
Q

What are the discrepancies between protein and mRNA in cells?

A

mRNA levels do not always correlate with protein levels in a cell

6
Q

What are protein arrays used for?

A

Detection of biomarkers
Protein quantification in cell lysate
Protein: protein interactions
Antibody characterisation