How Biotechnology Works- Part 2 Flashcards Preview

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Flashcards in How Biotechnology Works- Part 2 Deck (13)
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1
Q

Who invented recombinant DNA technology in 1972

2 people

A

Stanley Cohen of Stanford Univ

Herbert Boyer of UCSF

2
Q

Recombinant DNA technology or genetic engineering is a method that allows the ____ _ ___ in a ___ ___ to form a _____ ___

A

combination of genes
Test tube
Hybrid DNA

3
Q

Allows the transfer of a specific gene for a desirable trait

____ _____ or ____ ______

A

Genetic engineering or modern biotechnology

4
Q

Tool are available (5)

Join DNA fragments using DNA _______
Cutting genes at specific sites by using _____ enzymes
Gel ___________
Synthesize DNA or RNA using DNA or RNA _______
Multiply DNA using ________ ___ ______ (1983)

A
Ligase
Restriction
Electrophoresis
Polymerase
Polymerase chaine reaction (PCR)
5
Q

___ and ____ (1972) demonstrated that ____ cuts at ______ site and produces cohesive or ____ ends

A

Mertz and Davis
EcoR1
specific
sticky

6
Q

Cutting genes at specific sites

Some restriction enzymes cut DNA to produce ___ or ___ ends
Some produce ___ ends

A

complementary; sticky

blunt

7
Q

Separates charged molecules based on charge (positive, negative, neutral) and size

A

Gel electrophoresis

8
Q

In gel electrophoresis, charged molecules migrate on a medium to which ___ ___ is applied

A

electric current

9
Q

In Polymerase chain reaction:

Amplifying parts of DNA by _____

Involves repeated ___ _______ at __ temperature, primer _____, and DNA _____

A

In vitro replication

DNA denaturation
High
Attachment
synthesis

10
Q

Nobel Prize in chemistry co-winner (1993)

A

Kary Mullis

11
Q

Filipino that is co-discoverer of DNA ligase

A

Dr. Baldomero Olivera

12
Q

Cohen developed a plasmid with only _ EcoR1 site, having deleted __ sites
Then when this plasmid (pSC101) is cut with EcoR1, it will open up forming a ____ DNA ___ with ____ ends

A

1, 12
Linear, duplex
Sticky

13
Q

What animal did Cohen-Boyer-Berg use for experiment

A

African clawed toad