Microscopy of blood cells Flashcards Preview

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Flashcards in Microscopy of blood cells Deck (5):

How to set up a light microscope?

1. focus image on lowest magnification
2. close field iris
3. focus condenser until you get a sharp image at the edge of the disc of light
4. open field iris until whole field of view is illuminated
5. adjust condenser iris, start with it fully open and close until the image only just starts to darken


What stain is used?

Haematoxylin and eosin (H&E)


What colour is haematoxylin?

Purple blue but binds to acidic components of cells so binds to DNA and shows up the nucleus


What colour is Eosin?

Pinkish and binds to protein components particularly in cytoplasm


Proportions of WBC
Neutrophils, lymphocytes, monocytes, eosinophils, basophils and young neutrophils

Neutrophils: 40% to 60%

Lymphocytes: 20% to 40%

Monocytes: 2% to 8%

Eosinophils: 1% to 4%

Basophils: 0.5% to 1%

Band (young neutrophil): 0% to 3%