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Flashcards in PCR Deck (8)
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1
Q

What PCR?

A

A biochemical process capable of amplifying a single DNA molecule into millions of copies in a short time

2
Q

What are the three steps of PCR?

A

Denaturation
Annealing
Extension

3
Q

Describe the denaturation stage of PCR?

A

dsDNA templates heated to separate the strands

4
Q

Describe the annealing phase of PCR

A

Short DNA molecules (primers) bind to flanking regions of the target DNA

5
Q

Describe the extension phase of PCR

A

DNA polymerase extends the 3’ end of each primer along the template strands

6
Q

At which temperature is PCR performed?

A

Stage 1 - 95
Stage 2 - 61
Stage 3 - 72

7
Q

What are the clinical applications of PCR?

A

Fingerprinting
Genetic diagnosis
Viral detection

8
Q

What is in the mastermix?

A

dNTP
MgCl
Reaction buffer
Taq polymerase