Protein Electrophoresis Flashcards Preview

Advanced Higher Biology: Unit 1- Cells And Proteins > Protein Electrophoresis > Flashcards

Flashcards in Protein Electrophoresis Deck (5)
Loading flashcards...
1
Q

How can gel electrophoresis separate proteins?

A

Using their charge and/or size/shape

2
Q

How does gel electrophoresis separate proteins?

A

It uses current flowing through a buffer to separate proteins. The gel acts as a sieve, separating the proteins.

3
Q

What is one form of protein electrophoresis ?

A

SDS-PAGE

4
Q

What happens during SDS-PAGE?

A

The proteins are denatured and given a uniform negative charge; this means that the proteins can be separated based on their size as they migrate towards the positive electrode. Small proteins travel further through the gel than large proteins

5
Q

What is different if you use a native gel?

A

The protein is not denatured before hand. This allows proteins to be analysed in their folded state