Spectophotometry and Electrophoresis Flashcards Preview

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Flashcards in Spectophotometry and Electrophoresis Deck (10):
1

What is electrophoresis

method of analysing molecules on the basis of charge, by measuring their migration in an electric field

2

What is spectophotometry?

Way of analysing molecules on basis of spetral properties

3

Definition of absorbance

Fraction of incident light absorbed by a solution which is measured

4

What are the measurements of abdorbance

Log10
light transmitted through blank solution/light transmitted through test solution

There will be a particular wavelength at which absorbance is at a maximum

5

What colour will maximum absorbance of a red solution be?

It will be in the green region as the colour of the solution is that of the remaining light which is transmitted

6

What does sodium dithionite do to haemoglobin? How does this affect the absorbance spectrum?

Removes oxygen therefore altering electron resonance properties of haem and changing the absorbance spectrum?

7

What is the beer lambert law?

The absorbance of a solution is proportional to the concentration of the absorbing solute and to the distance travelled by the light through the sample

8

What is the equation summarising the beer lambert law?

A = E x c x l
E is extinction coefficient for the substance measured
C is concentration
I is path length

9

What can the beer lambert law do?

Determine the concentration of haemoglobin in a sample using its results from spectophorometry

10

What does the sickle mutation do? And why does this have an effect?

Single change in aa at position 6 from glutamate to valine. Glutatmate is negatively charged but valine is uncharged