Spectophotometry and Electrophoresis Flashcards Preview

Practicals > Spectophotometry and Electrophoresis > Flashcards

Flashcards in Spectophotometry and Electrophoresis Deck (10):

What is electrophoresis

method of analysing molecules on the basis of charge, by measuring their migration in an electric field


What is spectophotometry?

Way of analysing molecules on basis of spetral properties


Definition of absorbance

Fraction of incident light absorbed by a solution which is measured


What are the measurements of abdorbance

light transmitted through blank solution/light transmitted through test solution

There will be a particular wavelength at which absorbance is at a maximum


What colour will maximum absorbance of a red solution be?

It will be in the green region as the colour of the solution is that of the remaining light which is transmitted


What does sodium dithionite do to haemoglobin? How does this affect the absorbance spectrum?

Removes oxygen therefore altering electron resonance properties of haem and changing the absorbance spectrum?


What is the beer lambert law?

The absorbance of a solution is proportional to the concentration of the absorbing solute and to the distance travelled by the light through the sample


What is the equation summarising the beer lambert law?

A = E x c x l
E is extinction coefficient for the substance measured
C is concentration
I is path length


What can the beer lambert law do?

Determine the concentration of haemoglobin in a sample using its results from spectophorometry


What does the sickle mutation do? And why does this have an effect?

Single change in aa at position 6 from glutamate to valine. Glutatmate is negatively charged but valine is uncharged