Flashcards in Spectophotometry and Electrophoresis Deck (10):
What is electrophoresis
method of analysing molecules on the basis of charge, by measuring their migration in an electric field
What is spectophotometry?
Way of analysing molecules on basis of spetral properties
Definition of absorbance
Fraction of incident light absorbed by a solution which is measured
What are the measurements of abdorbance
light transmitted through blank solution/light transmitted through test solution
There will be a particular wavelength at which absorbance is at a maximum
What colour will maximum absorbance of a red solution be?
It will be in the green region as the colour of the solution is that of the remaining light which is transmitted
What does sodium dithionite do to haemoglobin? How does this affect the absorbance spectrum?
Removes oxygen therefore altering electron resonance properties of haem and changing the absorbance spectrum?
What is the beer lambert law?
The absorbance of a solution is proportional to the concentration of the absorbing solute and to the distance travelled by the light through the sample
What is the equation summarising the beer lambert law?
A = E x c x l
E is extinction coefficient for the substance measured
C is concentration
I is path length
What can the beer lambert law do?
Determine the concentration of haemoglobin in a sample using its results from spectophorometry