Sub-cellular Components

Question 1

the isolation of organelles by the use differential centrifugation

Answer 1

Subcellular Fractionation

Question 2

Steps in Subcellular Fractionation

Answer 2

1. Extraction
2. Homogenization
3. Centrifugation

Question 3

isolation of subcellular components by dropping specimen to sucrose or NSS

Answer 3

Extraction

Question 4

Preferred Concentration for Sucrose Sol’n

Answer 4

0.025 M

Question 5

Preferred Concentration for NSS

Answer 5

0.16 M

Question 6

Reason for using Sucrose and NSS to preserve sample

Answer 6

Because the molecules does not cross the cells

Question 7

the process of releasing the organelles inside the cells

Answer 7

Homogenization

Question 8

Types of Homogenization

Answer 8

Sonication
Detergent
French Press
Mechanical Homogenization

Question 9

a type of homogenization that uses sound waves

Answer 9

Sonication

Question 10

a type of homogenization that promotes the lysis of the cell to release the oragnelles

Answer 10

Detergent

Question 11

homogenization using pressure

Answer 11

French Press

Question 12

the use of blender to release the organelles

Answer 12

Mechanical Homogenization

Question 13

the process of separating the components by the use of centrifugal force at repeated and progressive speeds

Answer 13

Centrifugation

Question 14

The smaller the COMPONENT?

Answer 14

The higher centrifugal force required to sediment it

Question 15

Types of Supernatant

Answer 15

I - Nuclear Fraction
II - Mitochondrial Fraction
III - Microsomal Fraction

Question 16

I - Nuclear Fraction

Answer 16

• 10 mins
• Nucleic Acids
• DNA, RNA

Question 17

II - Mitochondrial Fraction

Answer 17

• 20 mins
• Mitochondria (CARBS)
• Lysosome and Golgi bodies

Question 18

III - Microsomal Fraction

Answer 18

• more than 40 mins
• microsome (LIPIDS AND PROTEINS)
• ribosome, cytoplasm, endoplasmic reticulum

Question 19

Different Qualitative Analysis for CARBS

Answer 19

BIURET
SUDAN IV
MOLISCH
DISCHE
BIAL/ORCINOL

Question 20

detects peptide bond by removing the nitrogen of the bonds and bonding it to the Cu2+ in the reagent

Answer 20

Biuret’s Test

Question 21

hydrophobic dye that stains lipids giving it a red color and sinks in polar substances or with the absence of lipids

Answer 21

Sudan IV

Question 22

general test for carbs that uses a-napthol and conc. H2SO4 to form a purple ring in the sample

Answer 22

Molisch Test

Question 23

used for the detection of DNA by using diphenlyamine to react with the 2-deoxypentose of the DNA

Answer 23

Dische Test

Question 24

used to detect pentose such as ribose by using 3,5-dihydroxytoluene creating a blue-green appearance

Answer 24

Orcinol/ Bial’s Test