Flashcards in T-Cell & MHC- Diebel Deck (38):
Do T-cells have to be present at the site to interact with an antigen?
YES!! They do not secrete their receptors
AND DO NOT recognize free antigen!!!
What are the main determinants of the precursor's cells ultimate progeny?
-conditions when dendritic cell was stimulated
-What TLR was engaged
-What cyotkines and chemokines predominant
What cytokines tell a niave T-cell to become Th1?
What is the main transcription factor that gets turned on once a cell becomes a Th1?
What cytokines to Th1 cells produce?
What cytokine tells a niave T-cell to become Th2?
What is the main transcription factor that gets turned on once a cell becomes Th2?
What cytokines tell a niave T-cell to become Th17?
What transcription factor is turned on to become Th17?
What cytokines do Th17 cells produce?
What cytokines tell a naive T-cell to become a ThFH?
What is the main transcription factor turned on in ThFH?
What cytokines to ThFH produce?
What cytokines tell a niave T-cell to become a Threg?
What is the main transcriptional factor turned on Threg?
What cytokines are produced by Threg?
What are the roles of Th1, and what do their cytokines do?
IFN gamma- pro-inflammatory, chemotatic agent for blood monocytes and tissue macrophages
Activate macs to become "classically activated M1" mac, avidly ingest or kill bacteria
T-cells recognize, macs remove
What is the biggest role of Th17 cells?
maintaining integrity of mucosal surfaces
Implicated in several auto immune diseases
What is the role of Th2 cells?
Alternatively activate Macs to M2
Helps wall off pathogens, and kill parasites by:
Chemotatic for eosionphils
Push B-cells to class switch from naive IgD/IgM to IgE
What is the role of ThFH?
to help B cells that have recognized antigen become activated an differentiate into antibody-secreting plasma cells
Where would you find cel surface markers:
CD3- all T-cells
CD8- T cytotoxic cells
CD4- T helper cells
Where would you find MHC class I molecules??
ALL NUCLEATED CELLS
-show proteins synthesized INSIDE the cell
Where would you find MHC class II molecules?
-show proteins endocytosed by the cell
What is cross presentation?
When a dendritic cell is able to express an antigen on MHC I and MHC II at the same time
What genetic variation to T cell receptors under go?
NOT somatic hypermutation
Describe positive and negative selection when it comes to T-cell maturation?
Positive selection - it needs to bind YOUR MHC molecules
Negative- it cannot cross react with any self antigens
What genes segments do the alpha and beta chains of T-cell receptors have?
Alpha chain has V, J, and C gene segments
Beta chain has V, D, J, and C gene segments
What chromosome is the MHC locus on?
Human Chromosome 6
How many different class I complex can one individual express?
(3 different gene loci, A, B, and C so one from each parent)
How many class II complexes can any one individual express?
DP (4 combos)
DQ (4 combos)
DR (4 combos)
What is MHC restriction?
T-cells are restricted to seeing antigens on MHC cells that are genetically identical to themselves
T-cell and APC must come from individuals who have the same alleles at a group of loci collectively called MHC
Diabetes type I, Grave's disease
Rheumatoid arthritis, diabetes type I