T-Cell & MHC- Diebel Flashcards Preview

IHO WEEK 3 > T-Cell & MHC- Diebel > Flashcards

Flashcards in T-Cell & MHC- Diebel Deck (38):
1

Do T-cells have to be present at the site to interact with an antigen?

YES!! They do not secrete their receptors

AND DO NOT recognize free antigen!!!

2

What are the main determinants of the precursor's cells ultimate progeny?

-conditions when dendritic cell was stimulated
-What TLR was engaged
-What cyotkines and chemokines predominant

3

What cytokines tell a niave T-cell to become Th1?

IL-12
IL-18
IFN gamma

4

What is the main transcription factor that gets turned on once a cell becomes a Th1?

T-bet

5

What cytokines to Th1 cells produce?

IFN gamma
TNF

6

What cytokine tells a niave T-cell to become Th2?

IL-4

7

What is the main transcription factor that gets turned on once a cell becomes Th2?

IL-4
IL-5
IL-13

8

What cytokines tell a niave T-cell to become Th17?

TGF-B
IL-1
IL-6
IL-23

9

What transcription factor is turned on to become Th17?

RORgammat

10

What cytokines do Th17 cells produce?

IL-17
IL-22

11

What cytokines tell a naive T-cell to become a ThFH?

IL-6
IL-21

12

What is the main transcription factor turned on in ThFH?

Bcl-6

13

What cytokines to ThFH produce?

IL-4
IL-21

14

What cytokines tell a niave T-cell to become a Threg?

IL-2
TGF-B

15

What is the main transcriptional factor turned on Threg?

FOXP3

16

What cytokines are produced by Threg?

IL-10
TGF-B

17

What are the roles of Th1, and what do their cytokines do?

IFN gamma- pro-inflammatory, chemotatic agent for blood monocytes and tissue macrophages

Activate macs to become "classically activated M1" mac, avidly ingest or kill bacteria

T-cells recognize, macs remove

18

What is the biggest role of Th17 cells?

maintaining integrity of mucosal surfaces

aggressively pro-inflammatory
Implicated in several auto immune diseases

19

What is the role of Th2 cells?

Alternatively activate Macs to M2

Helps wall off pathogens, and kill parasites by:
Chemotatic for eosionphils
Push B-cells to class switch from naive IgD/IgM to IgE

20

What is the role of ThFH?

to help B cells that have recognized antigen become activated an differentiate into antibody-secreting plasma cells

21

Where would you find cel surface markers:
CD3
CD8
CD4

CD3- all T-cells
CD8- T cytotoxic cells
CD4- T helper cells

22

Where would you find MHC class I molecules??

ALL NUCLEATED CELLS
-show proteins synthesized INSIDE the cell

23

Where would you find MHC class II molecules?

All APCs
-show proteins endocytosed by the cell

24

What is cross presentation?

When a dendritic cell is able to express an antigen on MHC I and MHC II at the same time

25

What genetic variation to T cell receptors under go?

Genetic recombination
Somatic mutation
NOT somatic hypermutation

26

Describe positive and negative selection when it comes to T-cell maturation?

Positive selection - it needs to bind YOUR MHC molecules

Negative- it cannot cross react with any self antigens

27

What genes segments do the alpha and beta chains of T-cell receptors have?

Alpha chain has V, J, and C gene segments

Beta chain has V, D, J, and C gene segments

28

What chromosome is the MHC locus on?

Human Chromosome 6

29

How many different class I complex can one individual express?

6
(3 different gene loci, A, B, and C so one from each parent)

30

How many class II complexes can any one individual express?

12
DP (4 combos)
DQ (4 combos)
DR (4 combos)

31

What is MHC restriction?

T-cells are restricted to seeing antigens on MHC cells that are genetically identical to themselves

T-cell and APC must come from individuals who have the same alleles at a group of loci collectively called MHC

32

B27

aknylosing spondylitis

33

DR2

narcolepsy

34

A3/B14

hemocrhomatosis

35

DG2/GQ8

Celiacs Disease

36

DR3

Diabetes type I, Grave's disease

37

DR4

Rheumatoid arthritis, diabetes type I

38

B53

protection against malaria