Testing For Biochemicals Flashcards Preview

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Flashcards in Testing For Biochemicals Deck (6)
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1
Q

What is the chemical test for protein?

A

To test for protein, a few drops of Biuret reagent are added to the solution and the mixture is gently shaken. It start as blue and a purple colour is a positive result.

2
Q

What is the chemical test for starch?

A

To test for starch, a few drops of iodine solution added to the solution, and the mixture is gently shaken. I starts as orange and a deep blue/black colour is a positive result.

3
Q

What is the chemical test for glucose (reducing sugars)?

A

To test for glucose, Benedicks reagent is added to the solution, and the mixture is heated in a boiling water bath. It starts as blue and an orange/Brick red colour is a positive result.

4
Q

Why might a doctor want to check for glucose?

A

A doctor might check a patients urine sample for glucose to find out whether he/she has diabetes (glucose is a positive result)

5
Q

How would you test for lipids?

A

The lipid can be mixed with water and ethanol and if a milky white emulsion is formed a lipid is present. Alternatively, you could rub the food on to dry filterpaper, and if it went from opaque to translucent, that would be a positive result

6
Q

How would you test for vitamins C?

A

You can add DCPIP and if the solution de-colourises, that you know that there is vitamin C. You can also count the amount of drops needed to find the relative concentrations of vitamin C.

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