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Flashcards in Yeast as an expression host Deck (8)
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1

What are the 4 types of yeast expression systems?

YIp
YEp
YRp
YCp

2

Describe YIp

Yeast integrating plasmids that integrate into the host genome

3

Describe YEp

Yeast episomal plasmids that are derived from endogenous yeast plasmids

4

Describe YRp

Yeast replicating plasmids that contain endogenous autonomous replicating sequences derived from the yeast chromosome

5

Describe YCp

Yeast centromere plasmids which contain a centromere from a chromosome and replicate like endogenous plasmids

6

What is good and bad about YRp and YRp?

High copy number but unstable

7

What is good and bad about YIp and YCp?

Low copy number but stable

8

Describe Pichia pastoris

Methyltrophic yeast
High expression
Easy scale-up
Inexpensive growth
Does protein folding and processing
Does post trans modifications
Has integrating plasmids that are designed to integrate the expression construct containing the desired gene and selectable marker into the host via recombination
Transormant is stable
Does N-glycosylation