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Felipe Sanches
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Decks in this class (53)
[01] Introduction to Molecular Biology
What is molecular biology,
What are the three key macromolec...,
What does dna stand for
20
cards
[02] DNA Structure and Replication
What is the structure of dna,
What are the components of a dna ...,
What are the four nitrogenous bas...
20
cards
[03] RNA Transcription and Translation
What is the first step in gene ex...,
Where does transcription occur in...,
What is the product of transcription
20
cards
[04] Protein Structure and Function
What is a protein,
What are the building blocks of p...,
What forms a peptide bond
22
cards
[05] Genetic Code
What is the genetic code,
How many nucleotides make up a codon,
What does each codon in the genet...
20
cards
[06] Gene Expression and Regulation
What is gene expression,
What are the two main steps of ge...,
What is gene regulation
24
cards
[07] Basics of Epigenetics
What is epigenetics,
What are epigenetic marks,
How do epigenetic changes affect ...
30
cards
[08] Primer Design
What is a primer in the context o...,
What is the purpose of a primer i...,
What are the key aspects to consi...
20
cards
[09] Polymerase Chain Reaction (PCR)
What is pcr,
Who developed the pcr technique,
When was the pcr technique developed
20
cards
[10] Gel Electrophoresis
What is gel electrophoresis,
What is the principle of gel elec...,
What is the gel in gel electropho...
21
cards
[11] DNA Sequencing
What is dna sequencing,
What is the significance of dna s...,
Name two common methods of dna se...
20
cards
[12] Introduction to Genomics and Proteomics
What is genomics,
What is proteomics,
What is a genome
20
cards
[13] Restriction Enzymes and Cloning
What are restriction enzymes,
What is the purpose of restrictio...,
How are restriction enzymes used ...
20
cards
[14] Plasmids and Vectors
What are plasmids,
What is the role of plasmids in b...,
Why are plasmids significant in g...
20
cards
[15] Tagging Proteins- HA, FLAG, and GFP
What are protein tags,
What is the purpose of tagging pr...,
Give examples of protein tags
11
cards
[16] Subcloning
What is subcloning,
What are the main steps of subclo...,
What is a restriction enzyme
20
cards
[17] Transformation and Transfection
What is transformation in genetics,
What organisms commonly undergo t...,
What is transfection
20
cards
[18] Bacterial Cloning
What is bacterial cloning,
What is the typical host organism...,
Why is e coli commonly used in ba...
20
cards
[19] Eukaryotic Cloning
What is eukaryotic cloning,
What is a common host organism us...,
Why is yeast commonly used in euk...
20
cards
[20] Cre-Lox Recombination
Define cre lox recombination,
What is cre in the context of cre...,
Define loxp
20
cards
[21] Transgenic Mice
1 what does the term transgenic mean,
2 define transgenic mice,
3 why are transgenic mice used in...
20
cards
[22] Construct Design for Cloning
What is the purpose of construct ...,
What elements are typically inclu...,
What is the role of a vector in a...
20
cards
[23] Mutagenesis
What is mutagenesis,
What are the two types of mutagen...,
What is site directed mutagenesis
20
cards
[24] Recombinant DNA Technology
What is recombinant dna technology,
What is the purpose of recombinan...,
What are the steps involved in re...
20
cards
[25] Southern, Northern, and Western Blotting
What is blotting,
What is southern blotting used for,
What is northern blotting used for
20
cards
[26] DNA and RNA Isolation Techniques
What is dna isolation,
What is rna isolation,
What are the general steps in dna...
20
cards
[27] Real-Time PCR (qPCR)
What is real time pcr qpcr,
What does qpcr stand for,
What makes qpcr different from st...
22
cards
[28] Reverse Transcription PCR (RT-PCR)
What is reverse transcription pcr...,
What does rt pcr stand for,
What is the key feature of rt pcr
20
cards
[29] DNA Microarray
What is a dna microarray,
What is the main application of d...,
What is gene expression profiling
20
cards
[30] Next Generation Sequencing
What is next generation sequencin...,
Ngs initials,
What are the key applications of ngs
20
cards
[31] Genome Editing (CRISPR, ZFNs, TALENs)
What is genome editing,
List the common genome editing te...,
What does crispr stand for
20
cards
[32] Cell Culture Techniques
What is cell culture,
List some types of cell culture t...,
What is monolayer culture
20
cards
[33] Mammalian Cell Culture
What is mammalian cell culture,
List the types of mammalian cell ...,
What is primary cell culture in m...
20
cards
[34] Bacterial Cell Culture
What is bacterial cell culture,
List the types of media used in b...,
What is nutrient broth used for i...
20
cards
[35] Fungal Cell Culture
What is a fungal cell culture,
What is the main purpose of funga...,
What are the basic requirements f...
20
cards
[36] Transient and Stable Transfection
What is transfection in molecular...,
What is transient transfection,
What is stable transfection
20
cards
[37] Immunoassays
What is the fundamental principle...,
What does an immunoassay involve,
Initials elisa
14
cards
[38] Immunocytochemistry (ICC)
What is the fundamental principle...,
Initials icc,
What are the main steps involved ...
11
cards
[39] Immunostaining
What does immunostaining refer to,
List the dyes used in immunostain...,
What is the nm range for the purp...
18
cards
[40] Diaminobenzidine (DAB)
What does dab stand for in the co...,
What is the primary use of diamin...,
What color does diaminobenzidine ...
6
cards
[41] Fluorescence In Situ Hybridization (FISH)
What does the acronym fish stand for,
What is the primary purpose of th...,
Is fish utilized in the field of ...
12
cards
[42] Immunohistochemistry (IHC)
What is the fundamental principle...,
Initials ihc,
What are the key steps involved i...
11
cards
[43] Cell Proliferation and Viability Assays
What is cell proliferation,
What is cell viability,
What is an assay
20
cards
[44] Apoptosis and Necrosis Assays
What is apoptosis,
What is necrosis,
What is the key difference betwee...
20
cards
[45] Protein Extraction and Purification
What is the purpose of protein ex...,
What are some common methods of p...,
What is the purpose of a protein ...
20
cards
[46] Immunoprecipitation
What is immunoprecipitation used ...,
What are the key components invol...,
What are protein a and protein g ...
20
cards
[47] Mass Spectrometry
What is the main purpose of mass ...,
What is the basic principle of ma...,
What is the m z ratio in mass spe...
20
cards
[48] Flow Cytometry
What is flow cytometry,
What can flow cytometry measure,
What is a typical application of ...
19
cards
[49] Confocal Microscopy
What is confocal microscopy,
How does confocal microscopy work,
What is a laser in confocal micro...
20
cards
[50] Bioluminescence and Fluorescence Assays
What is bioluminescence,
What is fluorescence,
How are bioluminescence assays us...
22
cards
[51] Radioactive Tracers in Molecular Biology
What is the principle of using ra...,
What are common isotopes used as ...,
Initials rt
10
cards
[52] Bioinformatics and Computational Biology
What is the focus of bioinformati...,
Initials bcb,
What types of biological data are...
12
cards
[53] Data Analysis in Molecular Biology
What is the main goal of data ana...,
Initials damb,
What types of data are commonly a...
10
cards
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[21] Introduction to Molecular Biology
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