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Ella Reshef
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Decks in this class (31)
1. DNA Structure and Properties
What is the difference between a ...,
What is the difference between pu...,
What is a chemical property of th...
19
cards
2. DNA Structure 2
Explain the basic structure of a ...,
Why are there major minor groves ...,
Where are the hydrgoen bonds in dna
5
cards
3. section 6 stability of DNA
What causes the stability of the dna,
What are the four ways dna can be...,
What are the effects of reduced s...
10
cards
4. section 7: size and shape of DNA molecules
What is supercoiling of dna,
Give three resasons why dna super...,
How does relaxed supercoiling occur
3
cards
5. Genes and Gene Expression - DNA replication
What is the semi conservative dna...,
How was radioactive isotopes of n...,
Dna is synthesised from normal pr...
12
cards
6. DNA replication 2
What is the function of dna pol 1,
What is the function of dna pol 2,
What is the function of dna pol 3
11
cards
7. DNA replication 3
Why are the 9 mer repeats on the ...,
Why are there so many a and t bas...,
What is dnaa
17
cards
8. DNA repair - safeguarding the genome
Define dna damage,
Define a mutation,
What is a subsitituation mutation
15
cards
8b DNA damage
What causes,
How does alkylation cause dna damage,
How do uv radiation
5
cards
9. Direct DNA repair systems
What is a feature of direct dna d...,
What enzyme,
What does the enzyme photolysase ...
5
cards
9b. Indirect DNA repair systems 1
What is a,
What is generalised,
What enzyme removes the base in g...
13
cards
9c. Indirect repair systems 2
What is,
What are the three steps for the ...,
What is mismatch repair
9
cards
10. Transcription 1
In dna transcription what does the,
What are the three parts of trans...,
Why is rna more suseseptible to b...
5
cards
11. Transcription 2
How was the,
What did ion exchange chromotogra...,
Is the protein concentration of r...
21
cards
12. the principles of gene regulation
What are the
1
cards
13. The Lac Operon of E Coli
In ecoli there is a lac operon wh...,
What is a promoter,
What is an operator
12
cards
15. The Tryptophan (Trp) operon
Define what attentuation is,
When is the trp operon turned off,
What is the trpr protein
9
cards
16. Riboswitches In The Control of Gene Expression
Define what a riboswitch is,
What percentage of gene expressio...,
Why are riboswitches important
12
cards
17. Intro to Recombinant DNA Technology
What is recombinant dna,
List the biochemical processes wh...,
Describe a plasmid and show how i...
4
cards
18 +19. Restriction Endonucleases
What are the four general tools u...,
What is a vector and how can it b...,
How are plasmids taken up by bact...
15
cards
19. Restriction endonuclease 2
What is the difference between t4...,
What is an isoschizomer and,
What
3
cards
20. Restriction endonuclease 3
How can blunt ends be made with t...,
What will the klenow enzyme do if...,
What will the klenow enzyme do if...
6
cards
21. E.coli transformation systems and systems for cloning
0
cards
21. E. Coli transformation & Selection systems for Cloning
Define competent cells,
How are competenet cells preparen...,
What is transformation
13
cards
22. Analysis of recombinant plasmids & PCR
How can an inserts orientation in...,
What is the pcr reaction,
What are the three requirements f...
13
cards
24. Making and screening genomic DNA
Why,
Define genomic libaries,
Define cdna libararies
9
cards
25. Constructing cDNA libraries
What are two sources of dna for d...,
Define cdna,
How does cdna libraries work
6
cards
26. Characterising genomic or cDNA libraries
What are the four techniques to c...,
What does southern blotting identify,
What are the 8 steps of southern ...
7
cards
29. Applications of Recombinant Technology
What is one important reason we w...,
What are two examples of recombin...,
What are four important par
8
cards
30. Applications 2 - Detecting differences in Peoples DNA
What is genetic variation,
What are the four sources of gene...,
What are snps
9
cards
30b. Applications
What does southern blotting tell ...,
What is rflp,
What does fish tell you about gen...
6
cards
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genes and gene expression
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