Flashcards in 18.01.08 Splicing Deck (5)
When and where does splicing occur?
In the nucleus pre-mRNA is processed to form mature RNA following 5' capping, 3' polyadenylation and splicing.
Removal of introns by endonucletolytic cleavage and ligation of exons.
How is splicing mediated?
By the major and minor spliceosomes; complexed of snRNA molecules and >100 proteins.
Major snRNAs: U1 U2 U4 U5 and U6
Minor snRNAs: U11 U12, U4atac, U5 and U6atatc
What is the GT-AG rule
Introns begin with GT (GU) and end with a AG.