Flashcards in 18.01.08 Splicing Deck (5)
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1
When and where does splicing occur?
In the nucleus pre-mRNA is processed to form mature RNA following 5' capping, 3' polyadenylation and splicing.
2
Summarise splicing.
Removal of introns by endonucletolytic cleavage and ligation of exons.
3
How is splicing mediated?
By the major and minor spliceosomes; complexed of snRNA molecules and >100 proteins.
Major snRNAs: U1 U2 U4 U5 and U6
Minor snRNAs: U11 U12, U4atac, U5 and U6atatc
4
What is the GT-AG rule
Introns begin with GT (GU) and end with a AG.
5