Jojo's Tube Guide

Question 1

What additives are in the gold top and the red/gray speckle top tube?

Answer 1

Clot activator and gel for serum separation. Inversión at time of blood collection is 5x’a

Question 2

What additive is in light green and green/gray speckle top tubes?

Plasma sample
Clue: light = additive starts with L

Answer 2

Lithium heparin and gel for plasma separation. Inversión at blood collection x’s 10

Question 3

What additive is in glass red top tube?

Answer 3

The glass red top tube is silicon coated. Inversión at blood collection is 0
It doesn’t need a clot activator because glass doesn’t prohibit clotting

Question 4

What additive is in red top plastic tube?

Answer 4

Red top plastic tube is silicon coated and has a clot activator. Inversión at blood collection is 5 x’s.

Question 5

What additive is in an orange top tube?

Sometimes referred to as RST Tubes (rapid Separator Tube).

Answer 5

Additive is thrombin based clot activator, with gel for serum separation
Inversions at blood collection 5 x’s

Question 6

What additive is in Navy blue top tube?

Answer 6

Additive is K2EDTA (di-potassium
Ethylenediaminetetraacetic acid)
INVERSIÓN IS 8 - 10x’s

Question 7

If sodium heparin is the additive what would be the tube colour?

Answer 7

Dark Green

Inversión at blood collection is 8 -10x’s

Question 8

If Lithium Heparin is the additive what would be the tube colour?

Answer 8

Light green

Inversión is 8 - 10 x’s

Question 9

These 3 additives can all be in the same colour tube
1. Potassium oxalate/sodium fluoride
2. Sodium fluoride/Na2 EDTA
3. Sodium fluoride ( serum tube)
What is the tube colour?

Answer 9

Gray
Inversión 8 - 10 x’s
Plasma sample

Question 10

Light yellow top tubes can have one of two types of additives, name them both?

Answer 10

1. Sodium polyanethol sulfonate ( SPS )
   OR
2. Acid citrate dextrose additives (ACD)

Question 11

You need to get a blood specimen for microbiology, they only want one yellow tube. What tube will you use?

Answer 11

Yellow tube with sodium polyanethol sulfonate (SPS)

This is for blood culture specimen collections microbiology.

Question 12

Liquid K2EDTA IS IN WHAT COLOUR TUBE?

Answer 12

A glass lavender top tube

Di-potassium Ethylenediaminetetraacetic acid

Question 13

What’s in a plastic lavender tube?

Answer 13

K2EDTA spray coated on the tube.

Inversión is 8 x’s

Question 14

A white tube has the same additive as a lavender tube. What is the difference between white and lavender tube?

Answer 14

The white tube also contains a gel for plasma separation. Inversión is still 8 x’a

Question 15

Pink has the same Additive as Lavender and white, what departments require a pink top tube?

Answer 15

Pink can be used for whole blood hematology.
immunohematology testing
blood donor screening.
Designed with special cross-match label for patient information. Tube inversions 8 - 10 x’s

Question 16

This tube requires 3-4 inversions, name the colour of this tube and it’s additive?

Answer 16

Light blue sodium citrate tube

Question 17

Exceptions for changing order of draw?

Answer 17

When there is a large no. Of one kind of tube, it may be advisable to draw one of each colour before drawing the group.
If it appears there is going to be difficulty obtaining all of the requests.if the flow stops early the phlebotomist has a better chance of more tests getting done.

Question 18

How does order of draw differ for micro container tubes?

Answer 18

1. Lavender is first because of EDTA. And to prevent specimen clotting.
2. Green
3. Gray
4. Gold
5. Red

Question 19

Why do you gently invert tubes rather than shaking?

Answer 19

Gently invert to ensure thorough mixing of the additives. Shaking the tube can cause haemolysis

Question 20

Why is the light blue tube drawn before serum tubes?

Answer 20

To prevent contamination with clot activators and interference with the coagulation cascade.

Question 21

What serum tubes are coated with clot activator?

Answer 21

Plastic serum tubes, because plastic tubes do not promote coagulation.

Question 22

What department and tests are done on gold top or red/grey speckle top tubes containing clot activator and gel for serum separation?

Answer 22

Immunology and serology dept used for chemistry determinations.

Question 23

What department and tests are performed on a light green (mint) or green/ gray speckle top tube? Containing lithium heparin and gel for plasma separation.

Answer 23

Usually referred to as a PST TUBE (plasma separation tube). Biochemistry department for tests that require plasma

Question 24

What department requires a red top glass tube that is silicon coated?

Answer 24

Used in biochemistry, immunology, serology and blood bank depts

Question 25

Gold top tubes and orange top tubes both provide serum for testing how do the differ

Answer 25

Gold are referred to as SST TUBES (serum separator tubes) orange top are referred to as RST TUBES (rapid separator tubes)

Question 26

What department and tests are performed on navy tubes (royal blue)? They contain K2EDTA
(Di-potassium Ethylenediaminetetraacetic acid)

Answer 26

Mainly used in toxicology dept, trace metal analysis and nutritional studies

Question 27

What department requires a dark green tube containing sodium heparin and what tests are done?

Clue: The testing laboratory requires living cells to provide a good cell culture.

Answer 27

Cytogenetics used mainly for cytogenetic testing and amino acids

Since all heparin tubes are colored some shade of green, it is important that one must read the additive written on the tube to insure that you are using Sodium Heparin and NOT Lithium Heparin. Lithium Heparin will not keep the cells alive long enough to yield a proper cell culture;

Question 28

Grey tubes containing potassium oxalate and sodium fluoride are for what tests?

Answer 28

Glucose and lactate
GGT - Gamma-glutamyl transferase

GGT levels fall after meals; you may be instructed to fast (nothing to eat or drink except water) for at least 8 hours prior to the test. You may also be asked to stop drinking alcohol or taking certain prescription medications.

Question 29

What additives are in grey top tubes

Answer 29

1. Potassium oxalate/sodium fluoride or
2. Sodium fluoride/Na2 EDTA
   Or
3. Sodium fluoride ( serum tube)

Question 30

What three places can you find serous fluid?

Answer 30

The pleural cavity (lung lining)
The pericardial (heart lining)
The peritoneal cavity (abdomen lining)

Question 31

What is effuson?

Answer 31

An increase in fluid volume in the pleural, pericardial and peritoneal cavities of the body. Usually caused by infection.

Question 32

What can cause hemoconcentration?Hemoconcentration is a decrease in plasma volume, which causes a simultaneous increase in the concentration of red blood cells and other commonly tested constituents of the blood.

Answer 32

1) Drawn blood from a patient who had gone from laying down to sitting upright immediately prior to the draw?
2) Left the tourniquet on for more than 60 seconds?
3) Instructed the patient to pump his or her fist?

Question 33

Vitamin K deficiency

Vitamin K is a necessarily component in factors II, VII, IX, and X. What will a deficiency in vitamin K lead to?

Answer 33

vitamin K will lead to a decrease in factors II, VII, IX & X an elevated PT. Potential causes that can lead to decreased vitamin K levels include malnutrition, prolonged antibiotic use, and impairments with fat absorption.

Question 34

What is a biosafety cabinet or fume hood and what does it do?

Answer 34

A biosafety cabinet or fume hood should eliminate exposure to volatile liquids, dusts, and mists. It is advisable to use a laboratory hood when working with all hazardous substances.