What is magnification?
The ratio of image size to object size (size of image/size of object).
What is resolution in microscopy?
The ability to distinguish between two objects that are close together.
What is the magnification range of light microscopes?
1000-2000x.
What is the resolution range of light microscopes?
50-200 nm.
What are light microscopes used for?
Viewing cells and tissues.
True or False: Light microscopes can view living things.
True.
What is the magnification range of scanning electron microscopes?
50,000-500,000x.
What is the resolution range of scanning electron microscopes?
0.4-20 nm.
What are scanning electron microscopes used for?
Viewing the surface of cells and organelles, and providing depth in three dimensional images.
What is the magnification range of transmission electron microscopes?
300,000-1,000,000x.
What is the resolution range of transmission electron microscopes?
0.05-1.0 nm.
What are transmission electron microscopes used for?
Detailing organelle ultrastructure.
How do scanning electron and transmission electron microscopes compare to light microscopes?
They have better resolution, allowing for more detail in magnified images.
What advantage do scanning electron microscopes provide?
3D images with depth of field.
What is a limitation of electron microscopes?
They require dried samples, which are therefore dead.
What does the term ‘artefact’ refer to in microscopy?
Changes in the shape of features seen due to dried samples.
How are images from electron microscopes typically presented?
In black and white, with colors added later by computer graphics.
What is the magnification range of laser scanning confocal microscopes?
1000-2000x.
What is the resolution range of laser scanning confocal microscopes?
50-200 nm.
What are laser scanning confocal microscopes used for?
Producing three dimensional images with good depth selection.
Fill in the blank: Laser scanning confocal microscopes can focus at a specific _______.
[depth].
True or False: Laser scanning confocal microscopes can only see dead specimens.
False.
What does a laser scanning confocal microscope rely on to create images?
A computer to piece together information from dots of light.
