CVS Sanpling

Question 1

What cells make up CVS?

Answer 1

CVS is placental tissue made up of trophoblasts (outer cells) & mesenchyme cells (inner).

Short term cultures (cyto trophoblasts), long term (mesenchyme cells).
Direct DNA extraction = cytotrophoblast

NB: Mesenchymal core culture results are more likely to reflect true fetal mosaicism than direct preparation.

Question 2

Risk of miscarriage from CVS?

Answer 2

1-2% (RCOG). Can lead to infection & or leakage of A/F.

Question 3

What gestation is CVS sampling carried out?

Answer 3

First trimester test: 11 to 13 wk gestation.

Question 4

What % of CVS samples have CPM?

Answer 4

~1.5-2% have confined placental mosaicism.