1
Q
What is a transgenic animal?
A
additional genes are introduced
2
Q
How are transgenic mice created?
A
Fertilised egg prior to fusion of the pronuclei. Use microinjection to insert foreign DNA. Eggs transferred into foster mother Analyse offspring for presence of transgene (PCR) Breed positive mice to generate homozygous mice
3
Q
Give 3 uses of transgenic animals
A
Model of disease
Insect control
Alter characteristics
4
Q
What is the chord mouse experiment?
A
Transgenic mouse expressing green fluorescent
5
Q
What are knockout mice?
A
In these animals a wild type gene is replaced with an inactive gene to create a “gene knockout”.
6
Q
What is the difference between transgenic and knockout?
A
T: Exogenous DNA inserted into random non-homologous site
K: Insertion of modified DNA at a specific homologous site
7
Q
How are knockout mice created?
A
Culture of ES cells
