LAB MANAGEMENT (BSC)

Question 1

Enclosed workspaces with a ventilated hood designed to contain pathogenic microorganisms during microbiological processes

Answer 1

BIOSAFETY CABINETS

Question 2

Primary purpose of BSC

Answer 2

Protect laboratory personnel and the environment from pathogenic microorganism, as aerosols might be formed during the processing of such microorganisms.

Question 3

T/F
BSC are only used for certain risk group organisms and for processes that might result in aerosol formation.

Answer 3

T

Question 4

decontaminate the air moving out of the cabinet

Answer 4

HEPA-filters

Question 5

Similarity of Laminar hood and BSC

Answer 5

Work as enclosed workspaces

Question 6

Difference of LAMINAR HOOD from BSC

Answer 6

Laminar hood: Only protects sample

BSC: Protects sample, personnel, environment

Question 7

Used BSC for BSL 1 and 2

Answer 7

I, II

Question 8

Used BSC for BSL 3 or 4

Answer 8

III

Question 9

BSC CLASS:

Protection: environment, personnel

Answer 9

I

Question 10

Most basic biosafety cabinet

Answer 10

I

Question 11

Open front. Unsterilized room air enters and passes through High Efficiency Particulate Air (HEPA) filter before being exhausted

Answer 11

I

Question 12

Typically used to either enclose specific equipment like centrifuges or for procedures like aerating cultures that might potentially generate aerosols.

Answer 12

I

Question 13

TYPES OF BSC CLASS I

Answer 13

Ducted: connected to the building exhaust system

Unducted: recirculating filtered exhaust back into the laboratory

Question 14

INFLOW VELOCITY OF BSC CLASS I

Answer 14

75

Question 15

• Room air is drawn in through the opening that also allows the entry of the operator’s arm during work.
• Air inside the cabinet then takes in the aerosol particles that may have been generated and moves it away from the operator towards the HEPA filter
• Air moving out of the cabinet is thus, sterilized via the HEPA filters before its discharge to the environment.
• Air moves in the front, towards the back, other recirculates within the working area before being exhausted from the HEPA filter at the top of the cabinet. Filtered air stays in the room.

Answer 15

CLASS I

Question 16

AIR EXHAUSTED OF BSC CLASS I

Answer 16

100

Question 17

AIR RECIRCULATED OF BSC CLASS I

Answer 17

0

Question 18

BSC CLASS
Protection: worker, environment, product

Answer 18

II

Question 19

An open-front, ventilated cabinet with vertical laminar flow. The air inside the cabinet is vented through a HEPA filter and recirculated through the cabinet. The exhaust from the cabinet is filtered through a HEPA filter.

Answer 19

II

Question 20

Involves a fan mounted in the top of the cabinet that draws a curtain of sterile air over the workstation where the biological products are being handled.

Answer 20

II

Question 21

Divided into 5 types depending on the: exhaust system and mechanism of work (recirculation of the exhaust air)

Answer 21

II

Question 22

Provides protection to the samples and environment, since make up air is also HEPA-filtered.

Answer 22

II

Question 23

Minimum required class in the laboratories.

Answer 23

II

Question 24

• Air moves underneath the work station and back up to the top of the cabinet before passing through the HEPA filters.
• The exhaust that moves out of the facility consists of air being drawn into the front of the cabinet underneath the work surface.
• Air drawn in acts as a barrier against the potentially contaminated air coming back out to the operator

Answer 24

II

Question 25

Not widely used (not safe to work with hazardous chemical subs.)

Answer 25

II – A1

Question 26

Contaminated air divided just above the work station and mixes with the inflow air

Answer 26

II – A1

Question 27

AIRFLOW VELOCITY OF CLASS II A1

Answer 27

75

Question 28

* Mixed air is drawn through a duct network so that it reaches the back of the cabinet * Air might be either recirculated after passing through the HEPA filters or exhausted out of the cabinet (also through a HEPA filter)

Answer 28

II – A1

Question 29

AIR EXHAUSTED OF CLASS II A1

Answer 29

30

Question 30

AIR RECIRCULATED OF CLASS II A1

Answer 30

70

Question 31

Not extensively used due to the chances of release of hazardous chemicals into the environment

Answer 31

II – A2

Question 32

What is protected in Class II A2 when the air enters the chamber through front aperture

Answer 32

operator/personnel

Question 33

If hazardous, volatile chemicals are to be used within the cabinet, along with the microbiological work, exhaust must be released into the atmosphere through the direct duct system

Answer 33

II – A2

Question 34

Inflow velocity of II – A2

Answer 34

100

Question 35

Inflow air mixes with the downflow air (from the top of the cabinet) and enters the front intake grille and then passes over the workstation where the air splits.

Answer 35

II A2

Question 36

60-70% of the contaminated air: recycled and pushed back into the workstation in the chamber through the downflow HEPA filter 30-40% of the contaminated air: exhausted through the exhaust HEPA filter

Answer 36

II A2

Question 37

Air exhausted by II A2

Answer 37

30

Question 38

Air recirculated by II A2

Answer 38

70

Question 39

uses single-pass airflow to control the flow of hazardous vapors

Answer 39

Type B cabinets (difference from type A)

Question 40

Has dedicated duct system which allows the release of the contaminated air out of the facility.

Answer 40

II– B1

Question 41

Has split downflow air – prevent buildup of chemicals within the cabinet.

Answer 41

II– B1

Question 42

Allows handling of volatile chemicals.

Answer 42

II– B1

Question 43

Inflow velocity of II B1

Answer 43

100

Question 44

Divide airflow so that the contaminated air is directed towards the exhaust system while the air between the operator and the workstation mixes with the inflow and is recirculated. Exhaust air dispersed out of the facility should be passed through the HEPA filters to provide protection to the environment.

Answer 44

II-B1

Question 45

Air exhausted by II-B1

Answer 45

70

Question 46

Air recirculated by II-B1

Answer 46

30

Question 47

aka “Total exhaust cabinet”

Answer 47

II – B2

Question 48

Similarity to Type A: air is drawn in from the front opening creating an air barrier that protects the operator

Answer 48

II - B2

Question 49

Advantage: removal of toxic vapors generated in the cabinet with no recirculation within the BSC Best to be used for tasks involving chemical vapor release For toxic and radionucleotides

Answer 49

II - B2

Question 50

Disadvantage: Expensive; limited to toxicology laboratories where protection against hazardous chemicals is imperative

Answer 50

II - B2

Question 51

Inflow velocity of II - B2

Answer 51

100

Question 52

* Air is drawn in from an opening at the top of the cabinet that supplies the downflow of air in the cabinet. * The air then passes through a HEPA filter, where 100% of the air is exhausted through a dedicated duct system with an exhaust fan motor. The air moving out of the facility is thus sterilized before its release into the atmosphere. * All of the contaminated airflow (100%) is externally exhausted which means the air drawn into the cabinet is 100% exhausted into the atmosphere. None of the air drawn into the BSC from either inflow or downflow is recycled within the airflow system (only passes once, no recirculation)

Answer 52

II - B2

Question 53

Air exhausted by II - B2

Answer 53

100

Question 54

Air recirculated by II - B2

Answer 54

0

Question 55

Similarity to Type B: working mechanism (however, these are designed to reduce operating costs & add flexibility to the laboratories)

Answer 55

II - C1

Question 56

Difference of Type C from Type A

Answer 56

Type C uses a single-pass airflow mechanism where air is not circulated.

Question 57

Difference of Type C from Type B

Answer 57

Type C • do not require a dedicated ducted exhaust system • can work for an extended duration to increase operator protection in the case of exhaust failure • run without the exhaust at all

Question 58

Inflow velocity of II - C1

Answer 58

100

Question 59

* Uses single-pass airflow system where cabinets move the air by mixing it with the downflow air separated into columns for recirculation. * Air above the workstation is drawn with a second fan which moves the contaminated air out through the exhaust system with a HEPA filter

Answer 59

II - C1

Question 60

Air exhausted by II - C1

Answer 60

70

Question 61

Air recirculated by II - C1

Answer 61

30

Question 62

Provide maximum protection to the operator, the environment, and the sample against high -risk group 4 pathogenic organisms

Answer 62

III

Question 63

A totally enclosed, ventilated cabinet. Laboratory workers manipulate items in the cabinet through attached rubber gloves that are accessible from outside the cabinet (this is why these cabinets are also termed ‘GLOVE BOXES’)

Answer 63

III

Question 64

Leak-tight, totally enclosed but ventilated cabinets, where all air that either enters or leaves through the facility pass through a HEPA filter

Answer 64

III

Question 65

Can be used for all four BSL (1, 2, 3, 4) but most important for manipulation of biological materials in the BSL 4 (i.e. Ebola virus)

Answer 65

III

Question 66

Mostly custom-built for specific laboratories with lab equipment build inside the chamber

Answer 66

III

Question 67

Has a transfer chamber that facilitates the sterilization of materials before they leave the glove box

Answer 67

III

Question 68

Provided with rubber gloves that are attached to the system to be used during operations in the cabinet.

Answer 68

III

Question 69

Inflow velocity of III

Answer 69

100

Question 70

Intake air is filtered through HEPA filters, and the exhaust air is filtered/treated through: double HEPA filters (arranged in a series) or HEPA filters in combination with incineration before being discharged outside of the building

Answer 70

III

Question 71

Air exhausted by BSC CLASS III

Answer 71

100

Question 72

Air recirculated by BSC CLASS III

Answer 72

0