outermost structure surrounding the bacterium;
cell envelope
cell envelope made up of
(cell wall, cell membrane and capsule)
found only in gram-negative bacteria, function as the cell’s initial barrier to the environment
outer membrane
portion of the cell envelope
located
external to the cell
membrane but internal to the capsule
cell wall (murein layer)
gives shape and rigidity found in nearly all clinically relevant bacteria;
glycocalyx
serves as the primary target of anti microbial agents
synthesis and function
gram characteristics
•gram positive cell wall
•gram negative cell wall
Cell Wall Functions:
a) Protection against mechanical disruption of the bacterial cell from adverse environmental conditions
b) Serves as a point of anchorage for flagella
c) Determines the staining characteristics of a species
Cell wall of Bacillus spp. contains
Cell wall of Mycobacterium spp. contains -
mycolic acid and waxy layer of glycolipids
Cell wall of fungi contains
chitin
lacks a rigid cell wall - pleomorphic and more sensitive to osmotic pressure
mycoplasma
ureaplasma
acholeplasma
3 layers of gram-positive bacteria
•peptidoglycan layer
•teichoic layer
•polysaccharide layer/glycoprotein later/capsule
Innermost layer made up of a very THICK PEPTIDOGLYCAN
(“aka” mucopeptide
or murein), teichoic acid
and
polysaccharide;
peptidoglycan layer
containing negatively charged teichoic acid (made up of repeating subunits of glycerol or ribitol phosphate and are covalently linked to the peptidoglycan) that contributes is to the charge of the cell wall
teichoic layer
an outermost layer outside the
peptidoglycan: helps protect the bacterium from the host immune system and can also be involved in
adhesion to surfaces or host cells.
Polysaccharide layer/Glycoprotein layer or Capsule
2 Layers of Gram Neg
inner layer
outer membrane
inner layer containing thin layer of ______ and a layer of _______
peptidoglycan
lipopolysaccharide
Outer membrane composed of
______, ________ and _______
proteins,
phospholipids
lipopolysaccharides
outer membrane also contains
porins (permeability)
periplasmic space (peptidoglycan
synthesis)
LPS contain three regions
•O-specific antigen
•polysaccharide core
•inner lipid A
main constituent of endotoxin
inner lipid A
gram negative bacteria contain
periplasmic space unlike gram pos bac