Flashcards in Practical 3 - Determination of RBC parameters Deck (8):
Percentage of volume of blood sample occupied by RBC
Mean Corpuscular Volume.
Hct / RBC count x 100
Mean Corpuscular Hb
Hb / RBC count
Mean Corpuscular Hb Conc
(Hb x 100/ RBC count)
What is used to do a red cell count?
How is HCT measured?
Blood drawn up via capillary action. Centrifuged in capillary tube.
After centrifugation, blood separates into plasma at top and Red and white cells at bottom
Ratio of length of Red cell column to total length of cells and plasma is Hct
How is Hb conc in the whole blood measured?
1. Hb released from RBC and converted to stable cyanmethaemoglobin.
2. Cyanmethaemoglobin then compared with standard using spectrophotometer
3. Unknown Hb conc = absorbance of test solution x conc of standard / absorbance of standard solution