Practical 4 - Enzyme Kinetics Flashcards Preview

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Flashcards in Practical 4 - Enzyme Kinetics Deck (3):
1

What artificial enzyme substrate is used for chymotrypsinogen?

GPNA.

GPNA hydrolysed forming N-G-L-P and p-nitroaniline.

2

At what wavelength does p-nitroaniline have a significant absorption?

410nm

3

What is the rate of enzyme catalysis?

Vo=Vmax x (S)/((S) + Km)

Vmax = max enzyme velocity

Km = substrate conc at which RoR is exactly half of that of the Vmax