practicals

Question 1

State the Beer-Lambert law

Answer 1

the absorbance of a solution is proportional to the concentration of the absorbing solute, and to the distance travelled by the light through the sample

A = E x c x l

Question 2

What is Vmax?

Answer 2

Maximum rate of reaction

The rate of reaction when the enzyme is saturated with substrate.

Question 3

What is Km?

Answer 3

the concentration of substrate which permits the enzyme to achieve half Vmax.
An enzyme with a high Km has a low affinity for its substrate, and requires a greater concentration of substrate to achieve Vmax.”