• Formation of sperm cell
• Due to expulsion of spermatozoa coming from the cloaca of frog
Spermatogenesis
inject urine subcutaneously
Male frog test / Galli Mainini test –
• Female frog test / Hogben’s test
• Refers to the extrusion of eggs within 8-12 hours
Oogenesis
Refers to the extrusion of eggs within 8-12 hours
Female frog test / Hogben’s test
Increased in blood flow that may lead to enlargement of uterus and ovary of animals
Hyperemia
- Use of female virgin rabbit
- Urine is injected at marginal ear vein
- (+) corpora hemorrhagica, hyperemic uterus
- Friedmann’s Test
Same as Friedmann’s except serum is used instead of urine
Hoffman’s Test
- Use of female mice
- Inject urine subcutaneously
- (+) corpora lutea, hemorrhagic follicle
Ascheim Zondek
- Use of female rats
- Inject urine subcutaneously
- (+) ovarian hyperfemia
Frank Bermann
- Use of female rats
- Inject urine intraperitoneally
- Ovarian hyperemia
Kupperman
- Identifies the presence of progesterone
- (+) formation of deep orange or dark brown color
- (-) amber
Prenandiol Test
- Presence of epinephrine
- (+) deep orange or dark brown
Iodine Test / Lugol’s Reaction Test
- The physician will administer Prostigmen intramuscularly, patient will wait for 2 weeks
- No bleeding = pregnant
- Bleeding = not pregnant
Prostigmen Withdrawal Bleeding Test
In Prostigmen Withdrawal Bleeding Test
No bleeding _______?
bleeding ______?
- No bleeding = pregnant
- Bleeding = not pregnant
Breast milk will be injected subcutaneously at the forearm, wait for 3 days
Skin Colostrum Test
In Skin Colostrum Test
No swelling or redness _____?
Swelling _______?
No swelling or redness = pregnant
- Swelling = not pregnant
The physician will give 5 tablets that will induce bleeding for 2 days
Primodos Oral
In Primodos Oral
- No bleeding within 5 days ________?
- Bleeding within 5 days ____________?
- No bleeding within 5 days = pregnant
- Bleeding within 5 days = not pregnant
