Recombinant technology continuation

Question 1

What is recombinant DNA technology

Answer 1

Question 2

List the 5 steps of Recombinant DNA technology

Answer 2

Protein identification
Gene isolation
Cloning and expression
Scale up Manufacturing
Quality assurance

Question 3

What is the objective of the protein identification step

Answer 3

Question 4

Expain the process of the protein identification step

Answer 4

Question 5

What is meant by redundancy of genetic code

Answer 5

many codons coding for the same amino acid

Question 6

What is the objective of gene isolation step

Answer 6

Question 7

List the three primary methods of gene isolation

Answer 7

Gene construction
mRNA isolation
DNA probing

Question 8

Explain gene construction

Answer 8

Question 9

Explain mRNA isolation

Answer 9

Question 10

Explain DNA probing

Answer 10

Question 11

What is the objective of gene cloning and expression

Answer 11

Question 12

Define gene cloning and expression

Answer 12

Question 13

List the key components of gene cloning and expression

Answer 13

Vector
rDNA molecule
Host cell
Master working cell bank

Question 14

Explain rDNA molecule as a key component for cloning and expression

Answer 14

Question 15

Explain vector as a key component for cloning and expression

Answer 15

Question 16

Explain host cell as a key component for cloning and expression

Answer 16

Question 17

Explain Master working cell bank as a key component for cloning and expression

Answer 17

Question 18

What is the objective of scale up manufacturing step

Answer 18

Question 19

List the 4 phases of sale up

Answer 19

Inoculum
Fermentation/ Cell culture
Purification
Formulation

Question 20

Explain inoculum

Answer 20

Question 21

Explain fermentation/ cell culture

Answer 21

Question 22

Explain purification

Answer 22

Question 23

Explain formulation

Answer 23

Question 24

What is the objective of Quality assurance

Answer 24

Question 25

Answer 25

Question 26

What are the 4 key areas of testing of quality assurance

Answer 26

Genetic material Bulk protein product Process validation Final product

Question 27

What is genetic material as quality assurance

Answer 27

Question 28

What is bulk production as quality assurance

Answer 28

Question 29

Explain process validation as quality assurance

Answer 29

Question 30

Explain final product as quality assurance

Answer 30

Question 31

What are the protein stability concerns

Answer 31