Tests + Materials

Question 1

Staphylococcus tests + incubation conditions + required materials

Answer 1

1. Coagulase Test (Staphaurex Plus)
2. DNase test
3. Mannitol salt agar & CHROMID S.aureus elite agar (SAIDE)
   Incubate 37C overnight

Materials:
1. Staphaurex Plus test kit (incl. Test reagent, Control reagent, card)
2. Staph aureus control
3. DNA agar plate
4. Staph epidermidis control
5. Mannitol salt agar plate x 3
6. CHROMID S.aureus Elite agar plate x2

Question 2

a-haemolytic Strep tests + required materials

Answer 2

1. Optochin susceptibility testing
   Incubate 37C overnight

Materials
1. Blood agar x3
2. Optochin disks x3
3. Controls = S. pneumoniae , S. viridans
4. Bijou x 3

Question 3

B-haemolytic Strep tests + required materials

Answer 3

1. Lancefield grouping
2. Bacitracin susceptibility test
3. MacConkey agar growth test.
   Incubate 37C overnight

Materials:
1. Oxoid Streptococcal Grouping kit (incl. enzyme extract x3 + latex reagents (A,B,C,D,F,G) + test card x3)
2. Wooden sticks
3. Blood agar plate x3
4. Bacitracin disks x3
5. Bijou x3
6. MacConkey agar plate
7. Controls = Strep. pyogenes, Enterococcus.

Question 4

y-haemolytic Strep tests + required materials

Answer 4

1. Lancefield Grouping test
2. Bile Aesculin growth test
   Incubate 45C overnight !!
3. MacConkey agar growth test
   Incubate 37C overnight

Materials:
1. Oxoid Streptococcal Grouping kit (enzyme extracts x3 + test reagents (A,B,C,D,F,G) + tests card x 3)
2. Bile Aesculin agar plates x 3
3. MacConkey agar plates x3
3. Controls = Enterococcus , Strep pyogenes

Question 5

Neisseria tests + required materials

Answer 5

1. Taxo Sugar Utilization test
   Incubate 37C 5-10% CO2 48hrs

Materials:
1. CHOCOLATE agar x2
2. Taxo sugar disks x2 (glucose (aka dextrose), maltose, lactose, sucrose)
3. Bijou x2
4. Control = N.lactamica.

Question 6

Moraxella tests + incubation + required reagents

Answer 6

1. DNase test
2. Catarrhalis disk test
   Incubate 37C 5-10% CO2 overnight.

Materials:
1. DNA agar plate x2
2. Catarrhalis disks x3
3. Controls = M. Catarrhalis & N.lactamica.

Question 7

Haemophilus tests + incubation + required materials

Answer 7

1. X and V growth factor tests
   Incubate 37C 5-10% CO2 overnight.

Materials:
1. DST agar x 3
2. X, V, XV disks x 3 each
3. Bijou x 3
4. Controls = H. parainfluenza + H. influenza

Question 8

Corynebacterium tests + incubation conditions + required materials

Answer 8

1. Trehalose fermentation
2. Urease production (& blood agar purity plate)
3. Tinsdale medium growth test.
   Incubate 37C for 48hrs.
   Remember to looses caps + dig wire into agar + aseptic technique!

Materials:
1. Trehalose broth x 2
2. Urea agar x2
3. Blood agar plate x2
3. Tinsdale medium agar plate x2
4. Control = C. ulcerans.

Question 9

Bacillus tests + incubation conditions + required materials

Answer 9

1. PEMBA agar growth test
2. Hanging drop motility test
   Incubate 37C for 48hrs

Materials:
1. PEMBA agar x 2
2. Vaseline, cavity slidex2, coverslip x2, broth culture of test + control
3. Control = bacillus cereus.

Question 10

Clostridioides tests + incubation conditions + required materials

Answer 10

1. Anaerobic Growth test
2. Metronidazole susceptibility test
3. Lactose Gelatin Medium test
4. Nagler reaction test.
   Incubate 37C 48hrs Anaerobic (+ aerobic)

Materials:
1. Blood agar x2
2. Metronidazole disk x2
3. Controls = C.perfringens type A, P.aeruginosa
4. Lactose Gelatin tubes x2
5. Controls = C.perfringens + C.sporogenes
4. Straight wire loop
5. Egg yolk agar x1
6. C.perfringens type A antitoxin.

Question 11

Bacteroides tests + incubation conditions + required materials

Answer 11

1. Anaerobic growth test
2. Metronidazole susceptibility test
3. MID8 Identification test
   Incubate at 37 anaerobically 48hrs (also aerobic)

Materials:
1. Blood agar x 4
2. Metronidazole disks x2
3. MID8 Identification mastring x2
4. Bijou x2
5. McFarland 0.5 standard
6. Controls = B. fragilis, P.aeruginosa.

Question 12

Pseudomonas tests + incubation conditions + required materials

Answer 12

1. Growth at different temperatures (4C/42C)
   Incubate at 4C for 72hrs.
   Incubate at 42C for 48hrs.
2. P.aeruginosa selective agar test.
   Incubate 37C for 48hrs.

Materials:
1. Blood agar x2
2. P.aeruginosa agar x2
3. Controls = P.aeruginosa, P.fluorescens.

Question 13

Enterobacterales tests + incubation conditions + required materials

Answer 13

1. Brilliance chromagar
2. Indole production
3. MR test
4. VP test
5. Dulcitol fermentation test
6. Lysine decarboxylase broth control
7. Lysine decarboxylase broth test
8. Phenylalanine agar
9. Urea agar
10. MacConkey agar purity plate
11. Citrate slope
12. API-20-E biochemical tests.
    Incubate 37C aerobic 24hrs.

Materials:
1. Sterile water in bijou
2. Tests above ^^
3. MacConkey agar x2
4. API-20-E test.
5. Mineral oil.