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HMS Chemical Biology and Biochemistry > Hemoglobin > Flashcards

Flashcards in Hemoglobin Deck (26)
1

Electron Transport Chain Outline

2

Venous Oxygen Delivery Outline

3

Oxygen consumption resting vs exercising

In an individual at rest, about 25% of the total oxygen is consumed, so that the remaining hemoglobin in the venous circulation is 75% saturated with oxygen. During exercise, when oxygen consumption increases, the cardiac output increases (due to a faster heart rate as well as more blood pumped with each beat). Furthermore, the degree of oxygen extraction is greater, so that the venous oxygen saturation is decreased. With intense exercise, up to 90% of the oxygen can be extracted.

4

Room air composition vs alveolar air composition

Note that under STP, alveolar air has a reduced oxygen content due to increased carbon dioxide and water vapor partial pressures.

5

Henry's Law

The amount of dissolved gas is proportional to the partial pressure

 

[O2] = aO2 * PO2

 aO2 = 0.0031 mL O2 x mmHg-1 x dL-1

where aOis the solubility constant of molecular oxygen.

6

Convention for gas dissolution in blood and standard measure

As a convention, we will consider the amount of oxygen dissolved in 100 mL (1 deciliter or dL) of plasma, and express this in terms of the volume of dissolved gas. When you do the math, this yields a value of 0.32 mL of oxygen/dL of plasma.

This calculation is without hemoglobin.  In reality, 98.5% of the oxygen in the blood is bound to hemoglobin; only a small amount is directly dissolved.

7

An average adult human body contains _____ of blood.

An average adult human body contains five liters of blood.

8

Hemoglobin's oxygen capacity

One gram of hemoglobin can bind to 1.34 mL of oxygen

9

Resting bloodflow diagram

10

Why is the heart so vulnerable to reduced blood flow caused by narrowing of the coronary arteries?

Because the heart extracts a large fraction of available oxygen (70%) from a small portion of blood which passes through it.  If this supply is cut off, the organ may become rapidly hypoxic.

11

Exercising bloodflow diagram

12

Myoglobin O2 binding

13

Oxygen-Myoglobin Dissociation Curve

P50 = 2.75 mm Hg for myoglobin

14

Oxygen-Hemoglobin Dissociation Curve

P50 = 27 mmHg for hemoglobin

15

30-60, 60-90

At a PO2 of 30, hemoglobin is 60% saturated, and at a PO2 of 60, hemoglobin is 90% saturated.

16

Cytochrome C oxidase is most active at a PO2 of . . .

10 mm Hg

17

T and R state for hemoglobin

18

Facilitators of T state

2,3-bisphosphoglycerate (2,3-BPG):  Small, heavily anionic molecule which nests into hemoglobin and interacts with cations in the two beta chains to force a T conformation.

Acidic conditions:  Result in protonation of beta chain histidines which facilitate ionic interaction between the alpha and beta chains, again forcing a T state conformation.  (Remember that the lungs are typically around pH 7.4, while active metabolism in tissues may drop the pH closer to 7.0)

 

Also bear in mind that O2 binding moves these ions away from one another, weakening ionic interactions and contributing to cooperative binding.

19

Bohr Effect

20

Acidic stabilization of the T state

21

2,3-bisphosphoglycerate production

22

2,3-bisphosphoglycerate in the T state

23

Fetal Hemoglobin

24

Oxygen-Hemoglobin F Dissociation Curve

25

Why won't a pulse oximiter give a good reading if the patient is breathing hyperbaric or concentrated oxygen?

It does not account for the oxygen that is dissolved in solution, free of hemoglobin.  Under standard conditions, this is negligible, but if alveolar PO2 is high, it may become clinically relevant.

26

How can the kidneys affect blood oxygen content?

Kidneys may sense a decreased level of oxygen delivery and secrete erythropoietin from the juxtaglomerular cells, stimulating erythropoiesis and thus increasing the amount of hemoglobin available to transport O2.