Lecture 2

Question 1

concept of immunofluorescence

Answer 1

Question 2

how are antibodies introduced into the cells if they can’t cross membranes?

Answer 2

Question 3

indirect vs direct immunofluorescence

Answer 3

Question 4

what are some important variations on immunofluorescence

Answer 4

Question 5

characteristics of antibodies

Answer 5

Question 6

characteristics of the production of antibodies

Answer 6

Question 7

what is immunisation to make antibodies

Answer 7

Question 8

what happens if satisfactory immunostaining is obtained

Answer 8

Question 9

polyclonal vs monoclonal antibodies

Answer 9

Question 10

what are hybridomas

Answer 10

Question 11

myeloma cell line characteristics

Answer 11

Question 12

what do hybridoma cell lines allow for

Answer 12

Question 13

how are monoclonal antibodies prepared up until fusing with myeloma cells

Answer 13

Question 14

cloning of hybridoma cells

Answer 14

Question 15

how are monoclonal antibodies prepared after the fusion with myeloma cells

Answer 15

Question 16

protocol of production of monoclonal antibodies

Answer 16

Question 17

advantages of monoclonal antibodies

Answer 17

Question 18

where GFP naturally occur

Answer 18

naturally found in fluorescent jellyfish

Question 19

what are GFP fusion proteins ideal for

Answer 19

ideal for use in time-lapse imaging and photobleaching experiments in live cells

Question 20

construction of GFP tagged proteins

Answer 20

Question 21

advantages of GFP

Answer 21

Question 22

disadvantages of GFP

Answer 22