Lecture 3 Flashcards

(23 cards)

1
Q

what is digital wide field microscopy

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2
Q

what are digital images made up of

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3
Q

greyscale vs color images

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4
Q

how are dyes and filters chosen in fluorescence images

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5
Q

what do digital wide field microscopy images show?

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6
Q

why is confocal microscopy used preferentially above traditional microscopes?

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7
Q

confocal microscopy diagram

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8
Q

how is the specimen scanned in a confocal microscope

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9
Q

what are biological uses of confocal microscopes

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10
Q

what is photobleaching

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11
Q

what is FRAP and what can it be used for

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12
Q

how can diffusion of molecules be measured by FRAP

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13
Q

what other movement can be observed by FRAP

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14
Q

what are some examples of super resolution microscopy

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15
Q

what is resolution

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16
Q

what is the equation for resolution

17
Q

characteristics of near field microscopy

18
Q

characteristics of total internal reflection (TIRF) microscopy

19
Q

characteristics of single molecule tracking

20
Q

characteristics of STORM

21
Q

characteristics of PALM

22
Q

limitations of PALM/STORM

23
Q

what are some other super resolution techniques