Lecture 3 - Plant Transformation - Casson Flashcards Preview

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Flashcards in Lecture 3 - Plant Transformation - Casson Deck (8)
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1

Why is breeding a genetics problem?

You must understand linkage. Unwanted traits may be inherited too.
It is also a numbers game - the more you can screen, the higher chance you will ultimately generate the plant you want.

2

What are the three main techniques of introducing foreign DNA into plants?

1. Vector (Agrobacterium) - mediated
2. Direct methods (gene gun)
3. Pollen magnofection

3

What are the three critical steps to make a GM plant?

1. Introduction of the construct (using any of the techniques)
2. Selection of transformed cells
3. Regeneration of a whole plant

4

What is the principle behind plant regeneration?

Regeneration of a whole adult plant from tissue taken from a donor

5

What tissues can be used?

- Embryo culture
- Apical meristem (stem cells of plants)
- Leaf tissue (contains meristematic tissue)
- Cell culture eg suspension cultures or protoplasts

6

What is the problem?

Some plants are difficult to regenerate, eg cotton.

7

Describe the steps in plant regeneration (and conditions)

1. Induce proliferation - callus formation (auxin and cytokinin)
2. Induce shoot formation (auxin and CYTOKININ)
3. Induce root formation (AUXIN and cytokinin)
4. Transplant to soil

8

What are common methods of selection?

Antibiotic resistance
Herbicide tolerance