methods

Question 1

steps in prepping slides

Answer 1

fixation, dehydration, clearing, infiltration/embedding, sectioning, staining

Question 2

an acidic dye is _____charged and liks with a _____ charged tissue group.

Answer 2

negatively, postively

Question 3

a basic dye is a _____ charged and forms salt with ____ charged tissue group

Answer 3

positvely, negatively

Question 4

Feulgren stains used for ? Name the reagent

Answer 4

DNA, schiff

Question 5

Carbohydrates are stained using this

Answer 5

PAS

Question 6

Fats are stained with these 2

Answer 6

Oil red O and Sudan black

Question 7

Proteins are identified via this technique

Answer 7

immunocytochemistry

Question 8

basic stain, turns tissue blue/purple

Answer 8

Hematoxylin

Question 9

acidic stain, stains tissue pink

Answer 9

Eosin

Question 10

Hematoxllin used for these srtuctures

Answer 10

DNA/RNA

Question 11

Eosin stains these structures

Answer 11

proteins, side chains

Question 12

Stain for connective tissue Masson’s Mallory

Answer 12

Trichrome

Question 13

elastic fiber stain types

Answer 13

aldehyde fuchsin orcein, resorcin-fuchsin

Question 14

stain for reticular fibers and CNS, appear black

Answer 14

silver impregnanation

Question 15

embedding material

Answer 15

paraffin, methacrylate, cellodin, gelatin

Question 16

fixative agents

Answer 16

10% buffered formalin, glutaraldehyde, alcohol, osmic acid

Question 17

clearing agents

Answer 17

toulene, benzene, xylene

Question 18

routine laboratory microscop

Answer 18

light (bright field)

Question 19

study cytology or inside of cells

Answer 19

TEM

Question 20

study features of cells, 3-D

Answer 20

SEM

Question 21

study living tissue

Answer 21

phase or interfernce

Question 22

uses laser beanm sections cell and makes 3-d image of cell

Answer 22

Confocal scanning microscope