For donors and patients involves detection of D antigen on red cells
Rh typing
Second most important blood group system
Rh system
C, E, c, e
5 major Rh antigens in decreasing immunogenicity
D>c>E>C>e
Reagent used in RH typing (6)
Anti-D serum
Anti-c serum
Anti-E serum
Anti-C serum
Anti-e serum
Rh/Hr control
Specimen used in RH typing
Unknown blood sample
Place glass slide on the Rh viewbox for______
5 minutes
Surface temperature of Rh viewbox
45 - 50°C
Deliver_____ drop anti-serum on each portion and add____ drop of whole blood to each portion
1 drop
1 drop
Observe agglutination in Rh typing for____
2 minutes
For the donors and patient involves the detection of D antigen on red cells
Rh typing
Obtain a blood sample from
Skin puncture or available blood sample