Weak D

Question 1

Is necessary before a medical technologist reports the result as RH negative

Answer 1

Weak D antigen

Question 2

What is the use of weak D determination

Answer 2

To determine the presence or absence of the Du factor or weak D antigen on red cells

Question 3

Weak D can be recognized most reliably by an

Answer 3

Indirect antihuman globulin test

Question 4

Reagent used in Weak D (4)

Answer 4

Anti-D serum

Antihuman globulin sera

Normal saline solution (0.85% to 0.9% sodium chloride)

22% bovine serum albumin

Question 5

Specimen used in weak D

Answer 5

Anticoagulated blood

Question 6

Manner of reporting of anti-D

+

0

Answer 6

RH positive

RH negative / initial

Question 7

Negative reaction should be further tested for the presence of weak D with the use of

Answer 7

Antihuman globulin test

Question 8

Manner of reporting of indirect antihuman globulin test

0

+

Answer 8

Du negative

Du positive

Question 9

Specimen used for weak D antigen determination

Answer 9

Anticoagulated blood sample

Question 10

“U” means

Answer 10

Unknown

Question 11

“NC” mean

Answer 11

Negative control

Question 12

After final wash add_____of AHG

Answer 12

2 drops

Question 13

How many times of cell washing in weak D determination

Answer 13

3 times

Question 14

Incubate Anti-D at____for____

Answer 14

37 C for 15 minutes

Question 15

Centrifuge AHG at_____for___

Answer 15

3400 rpm for 15 seconds

Question 16

What is the purpose of adding combs control

Answer 16

To confirm validity for negative results