Trigger 3

Question 1

Which areas of the heart muscle are affected in HCM?

Answer 1

• left v and interventricular septum

- become thick and stiff due to collagen

Question 2

Where are HCM causing mutations likely to occur?

Answer 2

• myosin binding protein c = most common
• beta myosin heavy chain
• Cardiac Tnt/Tni = less common

Question 3

What causes arrhythomgenic right ventricular cardiomyopathy?

Answer 3

• indel mutations in desmosome proteins –> frameshift
• desmosomes dont function properly
• reduced structural integrity
• segmental loss of of right ventricle myocardium
• -> replaced with fibrotic-fatty tissue

Question 4

What type of immunohistochemistry is good for an antigen/protein in low abundance?

Answer 4

Indirect

• more than 1 secondary AB can bind to primary AB
• GREATER SIGNAL

Question 5

How do you prevent non-specific protein binding in immunohistochemistry?

Answer 5

• Block the slide by washing with serum