lab 3

Question 1

one of basic tools of modern biotechnology

Answer 1

DNA splicing

Question 2

what procedure are we using in this lab

Answer 2

gel electrophoresis

Question 3

are DNA fragments negatively or positively charged

Answer 3

negatively

Question 4

what percent agarose gel in TAE are we using

Answer 4

1%

Question 5

TAE

Answer 5

biological buffer that keeps the pH of the solution in a specific range
- Tris, Acetate, EDTA

Question 6

what are we using to insert DNA sample into gel

Answer 6

micropipette

Question 7

L microtube composition

Answer 7

uncut lambda DNA

Question 8

P microtube composition

Answer 8

Pstl restriction digest of lambda DNA

Question 9

E microtube composition

Answer 9

EcoRI restriction digest of lambda DNA

Question 10

H microtube composition

Answer 10

HindIII restriction digest of lambda DNA

Question 11

what bacteriophage will be used

Answer 11

lambda

Question 12

what DNA stain/dye will we be using

Answer 12

UView

Question 13

UView composition

Answer 13

• Bromphenol Blue
• Xylene Cylanol
• proprietary fluorescent DNA stain