LOs: 17-20 Flashcards Preview

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Flashcards in LOs: 17-20 Deck (32)
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31

20 Nucleic acid amplification testing (NAAT):

Examples
- Rapid TB diagnostic
- Multiplex PCR
- 16S rRNA sequencing

Advantages

Disadvantages

- Rapid TB diagnostic (developing world, high sensitivity)
- Multiplex PCR (in parallel, rapid, qualitative)
- 16S rRNA sequencing (primers for conserved sequences, detecs unknown pathogens)

rapid, specific, quantitative

cost, labor, technically challenging, contamination, slow if sample sent to reference lab

32

20 Mass Spectrometry:

Components

Examples
- PLEX-ID
- Vitek MS

Advantages

Disadvantages

i. vaporization (ion source)
ii. measure mass-to-charge ratio (mass analyzer)
iii. abundance of molecule (mass detector)

- PLEX-ID: measures PCR, distinguishes by nucleotides
- Vitek MS: measures proteins, distinguishes peaks

rapid, general, direct from patient sample

overnight, sample prep, expense, sensitivity