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Flashcards in Biotech Final - Lab 1 Deck (10)
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1

What is a homolog?

A gene related to a second gene by descent from a common ancestral DNA sequence.

2

An effective method for analyzing protein sequences is to examine _______ ________ between a protein of interest and a group of protein ________.

i) Conserved domains
ii) Homologs

3

What are conserved domains?

Conserved domains are recurring units in polypeptide chains (sequence and structure motifs).

4

The extent of similarity between conserved domains of protein homologs can be determined by what?

Comparative analysis

5

Using a CD search one can?

1) Locate functional domains
2) Establish evolutionary relationship among protein families
3) Interpret mutation studies
4) Make predictions on the structure and function of unknown structure

6

What does the CDD (conserved domain database) contain?

Sequence alignments that define the features that are conserved within each domain family.

7

The CDD serves as a classification resource that groups proteins based on?

The presence of pre-defined domains

8

CDD entries often name the domain family and describe the _______ of conserved residues in ______ and ________.

i) function
ii) binding
iii) catalysis

9

The CD search is designed to interact with the _____ databases to perform multiple _______ alignments and detailed statistical analysis using the ______ software.

i) NCBI
ii) sequence
iii) BLAST

10

What does the Blast software return?

E-values (expectation values), the more hits, the higher the value.

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