Biotechnology (W16) - Week 3,4 - Day 6 Protocol: Fixation of DNA to a membrane Flashcards Preview

Biotechnology > Biotechnology (W16) - Week 3,4 - Day 6 Protocol: Fixation of DNA to a membrane > Flashcards

Flashcards in Biotechnology (W16) - Week 3,4 - Day 6 Protocol: Fixation of DNA to a membrane Deck (3)
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1

Following transfer, the position of the gel slots was marked on your membrane with a soft _____ ______.

lead pencil

2

What was the purpose of placing the membrane in neutralization buffer and lightly shake it for 5 minutes at low speed?

Remove any agarose that might have adhered to the membrane, and to equilibrate the membrane.

3

What was done to ensure that DNA samples do not become unattached from the membrane in downstream applications?

UV-Cross linking

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