Biotech lab 3-staining SDS-PA gels-background Flashcards Preview

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Flashcards in Biotech lab 3-staining SDS-PA gels-background Deck (6)
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1

What stain will we be using?

Coomasie Brilliant blue

2

What is coomasie brilliant blue?

Aminotriarylmethane dye which forms strong complexes and stoichiometrically binds with proteins but does not bind tightly to the SDS-PAGE gel.

3

How are proteins separated by SDS-PAGE fixed?

Using either methanol or ethanol:glacial acetic acid then stained with coomasie blue.

4

How long is the gel immersed in the fixing solution?

30 minutes

5

The uptake of the dye is proportional to what?

The amount of protein.

6

How is un-incorporated dye allowed to diffuse?

In a de-staining step.

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