Flashcards in Biotech lab 3-staining SDS-PA gels-background Deck (6)
What stain will we be using?
Coomasie Brilliant blue
What is coomasie brilliant blue?
Aminotriarylmethane dye which forms strong complexes and stoichiometrically binds with proteins but does not bind tightly to the SDS-PAGE gel.
How are proteins separated by SDS-PAGE fixed?
Using either methanol or ethanol:glacial acetic acid then stained with coomasie blue.
How long is the gel immersed in the fixing solution?
The uptake of the dye is proportional to what?
The amount of protein.