Oncogenes Flashcards Preview

HMS Oncology > Oncogenes > Flashcards

Flashcards in Oncogenes Deck (5)
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1
Q

Soft Agar Assay for Colony Formation

A

3D culture of cells in soft agar medium for 21-28 days as test for malignancy.

Tests for anchorage independence.

2
Q

Nude mice

A

Nude mice carry an autosomal recessive mutation in the nu allele (Foxn1) on chromosome 11 that abolishes proper thymus development and also renders the mice without hair. Have no functional T cells but B cells are normal.

3
Q

SCID mice

A

Severe Combined Immunodeficiency. Autosomal or X-linked recessive. Without bone marrow transplant, fatal by age 1-2 in humans.

Mutations in RAG-1 or RAG-2 are typical. Cause loss of formation of both T and B lymphocytes.

4
Q

Xenograft testing

A

Tumors may be transferred to Nude or SCID mice, who do not reject grafts, to see if they grow in this context.

5
Q

HER family RTKs

A