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Flashcards in Tumor Suppressor Genes Deck (18):
1

HPVs

Cause warts and papillomas and are associated with some specific cancers.  Have specific tropism for squamous epithelial cells (particularly keratinocytes).

 

 

2

Feature of a cervical carcinoma pap smear

Rounder cells

Hyperchromatic nuclei

Nuclear-to-cytoplasmic ratio is high

Abnormal contours/grooves in nuclei

Mostly non-keratinized (blue-staining)

 

3

Low risk HPV strains

6 and 11

Poor p53 affinity of E6

4

Koilocyte

A squamous epithelial cell that has been transformed by a papillomavirus. Characteristics include perinuclear clearing and abnormally shaped nucleus.  Diagnostic for cervical papilloma virus infection.

5

Regions of the HPV genome

E (for early)

and L (for late)

6

HPV16 and HPV18 account for __% of cervical cancers

HPV16 and HPV18 account for 70% of cervical cancers

7

Key risk factors for cervical carcinoma in women

Sexual promiscuity, an early age of onset of sexual activity, and poor sexual hygiene conditions 

8

Features of a healthy pap smear

Polygonal-shaped cells

Low nuclear to cytoplasmic ratio

Mixture of keratinized (red-staining) and non-keratinized (blue-staining) cells

A few neutrophils

9

HPV E6 and E7 function

10

Vegetative viral DNA replication and the synthesis of the capsid proteins, occur only in the____ of a papilloma

Vegetative viral DNA replication and the synthesis of the capsid proteins, occur only in the fully differentiated squamous epithelial cells of a papilloma

11

E7 mechanism

12

When sequencing the genome of a tumor you are likely to find:

  Mutations that activate one or two oncogenes and loss of function mutations in several tumor suppressor genes.

 

13

US approved HPV vaccines

Gardasil-9

 

Protects against 9 strains of HPV

14

A single vaginal swave sample can be used for __ and __.

a single vaginal swave sample can be used for cytopathology and HPV testing

15

HeLa cell line is. . .

An HPV18-positive cell line

16

Protection from the HPV vaccine is reduced by ~___% overall if it is given to a non-naive patient.

Protection from the HPV vaccine is reduced by ~50% overall if it is given to a non-naive patient.

 

Likely partially due to proviral DNA integration

17

E6 Mechanism

18

L1 and L2

"Late" papilloma genes.  Expressed exclusively in the terminally differentiated keratinocytes at the surface of the skin.  These are the capsid proteins for HPV.