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Flashcards in Forensic DNA database Deck (6)
1

What is CODIS?

Combined DNA Index System

CODIS is the FBI's program of support for criminal justice DNA databases as well as the software used to run these databases.

2

How many core loci underly CODIS and how many population datasets are included?

13 core loci

over 50 population databases; more than 10 000 individuals

3

What does YHRD stand for?

Y-STR Haplotype Reference Database

4

How many core loci are defined by SWGDAM for Y-STR analysis?

11

5

SWGDAM recommendations for mtDNA typing

  • GLP and follow specific protocols
  • Negative and positive controls and extraction reagent blanks
  • reported sequences must be based on redundant information from forward and reverse primers
  • manual transcription should be avoided
  • independent confirmation by two scientists
  • labs shall participate in proficiency testing programs
  • sequence entire mitochondrial DNA control region in population genetic studies
  • report relative to rCRS, allignment needed
  • use IUPAC convention
  • use analytical software that checks for phylogenetics
  • be able to justify choice of database and statistical approach
  • establish statistical guidelines for reporting match between two samples

6

SWGDAM recommendations for mtDNA heteroplasmy

  • labs have to establish their own guidelines on how do deal with them
  • "differences in both PHP and LHP do not constitute evidence for excluding two otherwise identical haplotypes as deriving from the same source or same maternal lineage"
  • put the dominant variant into database