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Flashcards in Animal cloning Deck (5)
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What is differentiation?

Developmental events between fertilised egg and mature organism and the specialisation of cells for functions


Describe Dolly the sheep

Nuclear transfer- remove nucleus from recipient egg and inject donor cell and fuse together


Describe xenotransplantation

Transplantation of animal organs into humans
Possible to manipulate genes involved in rejection


Describe the process of gene knockout by nuclear transfer

Alpha 1,3-galactosyltransferase is not present in primates and it adds a carbohydrate antigen to pig cells which is attacked by the human immune system
Knockout of this gene by HR and NT using cultured foetal fibroblasts provides a foundation for xenotransplantation


Describe iPs cells

Induced pluripotent stem cells
Reverse differentiation by administering 4 transcription factors in viral vectors