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BIO2030 Biotechnology > Animal cloning > Flashcards

Flashcards in Animal cloning Deck (5)
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1

What is differentiation?

Developmental events between fertilised egg and mature organism and the specialisation of cells for functions

2

Describe Dolly the sheep

Nuclear transfer- remove nucleus from recipient egg and inject donor cell and fuse together

3

Describe xenotransplantation

Transplantation of animal organs into humans
Possible to manipulate genes involved in rejection

4

Describe the process of gene knockout by nuclear transfer

Alpha 1,3-galactosyltransferase is not present in primates and it adds a carbohydrate antigen to pig cells which is attacked by the human immune system
Knockout of this gene by HR and NT using cultured foetal fibroblasts provides a foundation for xenotransplantation

5

Describe iPs cells

Induced pluripotent stem cells
Reverse differentiation by administering 4 transcription factors in viral vectors