Flashcards in Perfect and problematic expression Deck (5)
Why is there a protein solubility problem in E. coli?
The dissimilarity of eukaryotic proteins and E. coli post-translational processes
What can't E. coli do to a protein?
What happens to the proteins produced?
They are produced as insoluble deposits in the cell
Add denaturants to form linear polypeptides
Remove denaturants to refold into soluble proteins