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Flashcards in Library construction Deck (3)
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1
Q

What is a genomic library?

A

A collection of clones that include all of the DNA sequences of a given species that can be screened for any clones containing the sequence of interest

2
Q

How are cDNA libraries made?

A

From isolated mRNAs

3
Q

How is cDNA made?

A

Reverse transcriptase makes DNA from RNA

Linkers are added to the ends of the DNA to help it join to the vector