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Flashcards in Library construction Deck (3)
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What is a genomic library?

A collection of clones that include all of the DNA sequences of a given species that can be screened for any clones containing the sequence of interest


How are cDNA libraries made?

From isolated mRNAs


How is cDNA made?

Reverse transcriptase makes DNA from RNA
Linkers are added to the ends of the DNA to help it join to the vector