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Flashcards in Homologous recombination Deck (9)
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1

What is HR?

A single copy of the modified gene is modified in its normal chromosomal environment and allows for the natural repair mechanism of non-dividing cells to add the transgene

2

What cells are used in HR?

Embryonic stem cells
From the inner cell mass of the blastocyst
Can develop into any type of cell

3

Describe gene knockouts

A gene is inserted and expresses a marker gene in a position that results in a natural gene being inactivated

4

Describe gene knockins

Insert and expression of a gene

5

Describe a positive selection marker

The presence of a gene in the sequence that provides resistance and therefore survives

6

Describe a negative selection marker

The presence of a gene in the sequence results in cell death

7

What are the 2 ways of transfecting cells?

Electroporation
Lipofection

8

Describe electroporation

Expose cells to an electric field which briefly opens the pores in the cell membrane and allows for the DNA to pass-through
This can damage the cells

9

Describe lipofection

Cationic lipids form liposome vesicles with DNA and then bind with the cell membrane